Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	91,323	G→A	E98K (GAA→AAA)	ftsL →	cell division protein FtsL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	91,323	0	G	A	100.0%	102.5 / NA	27	E98K (GAA→AAA)	ftsL	cell division protein FtsL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/14);  total (13/14)

GAGCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAATAAGGA  >  NC_000913/91248‑91400                                                                            |                                                                              gAGCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAg                                                                           >  2:18483/1‑80 (MQ=255)   gCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGAt                                                                        <  1:18445/81‑1 (MQ=255)    cGAGATGCTTTAGACATTGAATGGCGCAACCTTATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATc                                                                       <  2:18446/81‑1 (MQ=255)    cGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATc                                                                       <  2:18447/81‑1 (MQ=255)       gaTGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGcc                                                                    <  1:18448/81‑1 (MQ=255)        aTGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCa                                                                   >  2:18487/1‑81 (MQ=255)        aTGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCa                                                                   <  2:18449/81‑1 (MQ=255)          gCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACg                                                                 >  1:18482/1‑81 (MQ=255)             ttAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGa                                                               >  2:18499/1‑80 (MQ=255)               aGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGaa                                                              >  2:18492/1‑79 (MQ=255)                gACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAg                                                           >  2:18489/1‑81 (MQ=255)                   aTTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTg                                                        >  1:18484/1‑81 (MQ=255)                   aTTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTg                                                        >  2:18474/1‑81 (MQ=255)                             gcAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATgcagc                                              <  2:18450/81‑1 (MQ=255)                                aCCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATg                                           <  2:18451/81‑1 (MQ=255)                                      tCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATc                                     <  2:18452/81‑1 (MQ=255)                                        cTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATcc                                    <  1:18453/80‑1 (MQ=255)                                             agagAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTcaca                              <  1:18454/81‑1 (MQ=255)                                              gagaATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTcac                               >  2:18493/1‑79 (MQ=255)                                               agaATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAg                            <  2:18455/81‑1 (MQ=255)                                                  aTGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGaaa                         <  1:18456/81‑1 (MQ=255)                                                  aTGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGaa                          >  2:18498/1‑80 (MQ=255)                                                     cgcTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAata                      <  1:18457/81‑1 (MQ=255)                                                                     ccGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAAt       >  2:18506/1‑80 (MQ=255)                                                                      cGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAAt       >  2:18495/1‑79 (MQ=255)                                                                        ggTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAATAAg    >  2:18505/1‑80 (MQ=255)                                                                         gTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAATAAGGa  <  1:18458/81‑1 (MQ=255)                                                                            |                                                                              GAGCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAATAAGGA  >  NC_000913/91248‑91400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑