Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,277,223	G→C	A132A (GCC→GCG)	narX ←	sensory histidine kinase NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,277,223	0	G	C	100.0%	123.2 / NA	33	A132A (GCC→GCG)	narX	sensory histidine kinase NarX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (10/23);  total (10/23)

GATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCAT  >  NC_000913/1277150‑1277296                                                                          |                                                                          gATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGcaaca                                                                     >  2:237033/1‑80 (MQ=255)  aTGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACaaa                                                                   >  2:237026/1‑81 (MQ=255)     gACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTg                                                                <  1:236995/81‑1 (MQ=255)      aCCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTgg                                                               <  1:236996/81‑1 (MQ=255)        cAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCt                                                             <  1:236997/81‑1 (MQ=255)         aGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTg                                                            <  2:236998/81‑1 (MQ=255)           tACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGAc                                                          <  2:236999/81‑1 (MQ=255)            aCCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACa                                                         >  2:237030/1‑81 (MQ=255)             ccACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACAt                                                        <  1:237000/81‑1 (MQ=255)                cTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCg                                                     <  2:237001/81‑1 (MQ=255)                  gTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCt                                                   >  1:237042/1‑81 (MQ=255)                       gATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGaca                                               <  2:237002/80‑1 (MQ=255)                            gcATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGttt                                         <  2:237003/81‑1 (MQ=255)                            gcATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGttt                                         <  2:237004/81‑1 (MQ=255)                              aTTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTtct                                       <  2:237005/81‑1 (MQ=255)                               tttCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTtctc                                      <  1:237006/81‑1 (MQ=255)                               tttCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTtctc                                      <  2:237007/81‑1 (MQ=255)                                  ccGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCgg                                    <  2:237008/80‑1 (MQ=255)                                     tggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGtttt                                <  2:237009/81‑1 (MQ=255)                                           ggTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCAc                          >  1:237056/1‑81 (MQ=255)                                             tCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGc                        <  1:237010/81‑1 (MQ=255)                                                  aCCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAg                   <  1:237011/81‑1 (MQ=255)                                                  aCCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAg                   <  1:237012/81‑1 (MQ=255)                                                     aGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAgcgc                <  1:237013/81‑1 (MQ=255)                                                     aGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAgcgc                <  2:237014/81‑1 (MQ=255)                                                        tACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAgg             <  2:237015/81‑1 (MQ=255)                                                            aGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATc         <  1:237016/81‑1 (MQ=255)                                                              ttGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAg       >  1:237046/1‑81 (MQ=255)                                                                gATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGtt     >  1:237039/1‑81 (MQ=255)                                                                gATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGtt     >  1:237038/1‑81 (MQ=255)                                                                gATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGtt     >  2:237043/1‑81 (MQ=255)                                                                 aTCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTc    <  2:237017/81‑1 (MQ=255)                                                                   cAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCAt  >  2:237041/1‑81 (MQ=255)                                                                          |                                                                          GATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCAT  >  NC_000913/1277150‑1277296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑