Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,284,681	T→C	S360P (TCT→CCT)	narH →	nitrate reductase A subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,284,681	0	T	C	100.0%	142.7 / NA	39	S360P (TCT→CCT)	narH	nitrate reductase A subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/14);  total (25/14)

CAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATT  >  NC_000913/1284604‑1284758                                                                              |                                                                              cAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGcctc                                                                            <  1:238430/81‑1 (MQ=255)   atggaatggaAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCg                                                                          <  2:238431/81‑1 (MQ=255)         tggaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAg                                                                    >  2:238489/1‑81 (MQ=255)           gaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTc                                                                  >  2:238507/1‑81 (MQ=255)            aaaCTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCt                                                                 >  2:238501/1‑81 (MQ=255)            aaaCTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCt                                                                 >  2:238482/1‑81 (MQ=255)             aaCTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTg                                                                >  2:238474/1‑81 (MQ=255)                  gcgcTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTgcag                                                             >  2:238477/1‑79 (MQ=255)                   cgctgccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGcagcag                                                          <  1:238432/81‑1 (MQ=255)                   cgctgccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGcagcag                                                          <  1:238433/81‑1 (MQ=255)                   cgctgccgctgcATCCGGAATATCGCACACTGCCGATGGTCGGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTgcag                                                             >  2:238494/1‑78 (MQ=255)                        ccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGAcgc                                                      <  2:238434/80‑1 (MQ=255)                             gcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGt                                                  >  1:238478/1‑79 (MQ=255)                              cATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTg                                                 <  2:238435/79‑1 (MQ=255)                                 ccGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTg                                            >  1:238504/1‑81 (MQ=255)                                  cGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTgg                                           >  2:238498/1‑81 (MQ=255)                                   ggAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTggg                                          >  1:238509/1‑81 (MQ=255)                                   ggAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTggg                                          >  1:238491/1‑81 (MQ=255)                                         tCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCaa                                    <  1:238436/81‑1 (MQ=255)                                            cacaCTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACgg                                 <  2:238438/81‑1 (MQ=255)                                            cacaCTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACgg                                 <  1:238437/81‑1 (MQ=255)                                              cacTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCa                               >  2:238492/1‑81 (MQ=255)                                               acTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCAt                              >  2:238502/1‑81 (MQ=255)                                               acTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCAt                              >  1:238484/1‑81 (MQ=255)                                                cTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCAtt                             <  2:238439/81‑1 (MQ=255)                                                      aTGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCa                       <  1:238440/81‑1 (MQ=255)                                                       tGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAg                      <  1:238441/81‑1 (MQ=255)                                                        ggTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGa                     >  1:238485/1‑81 (MQ=255)                                                                 cGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGaaa             <  2:238443/80‑1 (MQ=255)                                                                 cGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGaaa             <  2:238442/80‑1 (MQ=255)                                                                  gTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGc           >  1:238514/1‑81 (MQ=255)                                                                  gTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGc           >  1:238503/1‑81 (MQ=255)                                                                    gccgccTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCt         >  2:238486/1‑81 (MQ=255)                                                                      cgccTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGc       >  1:238513/1‑81 (MQ=255)                                                                       gccTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCg      >  2:238488/1‑81 (MQ=255)                                                                       gccTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCg      >  2:238519/1‑81 (MQ=255)                                                                        ccTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCg      >  1:238496/1‑80 (MQ=255)                                                                        ccTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGt     <  2:238444/81‑1 (MQ=255)                                                                         ctctGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTa    >  2:238490/1‑81 (MQ=255)                                                                           ctGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTAtt  >  2:238506/1‑81 (MQ=255)                                                                              |                                                                              CAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATT  >  NC_000913/1284604‑1284758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑