Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,497,760	A→C	D302A (GAT→GCT)	opgD →	glucan biosynthesis protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,497,760	0	A	C	100.0%	88.6 / NA	24	D302A (GAT→GCT)	opgD	glucan biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/15);  total (9/15)

CGATGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGATCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAGTTC  >  NC_000913/1497682‑1497833                                                                               |                                                                          cGATGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTc                                                                         >  1:274455/1‑81 (MQ=255)  gATGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCg                                                                        <  1:274422/81‑1 (MQ=255)  gATGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCg                                                                        >  1:274458/1‑81 (MQ=255)   aTGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGt                                                                       >  1:274435/1‑81 (MQ=255)      aCCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCt                                                                     <  2:274423/80‑1 (MQ=255)          gTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCa                                                                <  2:274424/81‑1 (MQ=255)             tGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCAtg                                                              <  1:274425/80‑1 (MQ=255)                tCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGc                                                          >  1:274452/1‑81 (MQ=255)                       cGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCaa                                                   >  2:274456/1‑81 (MQ=255)                            cTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCg                                              <  2:274426/81‑1 (MQ=255)                               aTGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGa                                             <  1:274427/79‑1 (MQ=255)                                  aaCGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGa                                        >  1:274459/1‑81 (MQ=255)                                   aCGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGAt                                       <  1:474473/81‑1 (MQ=255)                                      tCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTg                                    <  2:274428/81‑1 (MQ=255)                                           tgtgCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTc                               >  1:274453/1‑81 (MQ=255)                                           tgtgCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTc                               <  2:274429/81‑1 (MQ=255)                                               cGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCt                           <  1:274430/81‑1 (MQ=255)                                                 aTACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGa                         <  2:274431/81‑1 (MQ=255)                                                      aTTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGaataat                    <  1:274432/81‑1 (MQ=255)                                                         cATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATcc                  <  1:274433/80‑1 (MQ=255)                                                         cATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCg                 >  1:274467/1‑81 (MQ=255)                                                                   ttCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATTg        >  2:274451/1‑80 (MQ=255)                                                                      aTGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAg     <  1:274434/80‑1 (MQ=255)                                                                         aCTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAGTTc  <  2:274435/80‑1 (MQ=255)                                                                               |                                                                          CGATGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGATCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAGTTC  >  NC_000913/1497682‑1497833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑