Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,512,246	T→G	V198G (GTT→GGT)	ydcS →	putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,512,246	0	T	G	100.0%	103.2 / NA	28	V198G (GTT→GGT)	ydcS	putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/12);  total (16/12)

CCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCT  >  NC_000913/1512170‑1512318                                                                             |                                                                         ccGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTa                                                                        >  2:811932/1‑79 (MQ=255) ccGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTa                                                                        <  2:276744/79‑1 (MQ=255)   ggACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGc                                                                    >  1:276779/1‑81 (MQ=255)     aCGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCa                                                                  >  1:276778/1‑81 (MQ=255)          aaGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAg                                                             >  1:276789/1‑81 (MQ=255)                    aaGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTgg                                                   >  2:276784/1‑81 (MQ=255)                     aGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTggg                                                  >  1:276787/1‑81 (MQ=255)                      ggCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGc                                                 <  1:276746/81‑1 (MQ=255)                         cgcgTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCAt                                               >  2:276777/1‑80 (MQ=255)                             ttCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCg                                          <  2:276747/81‑1 (MQ=255)                                 ggCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATcc                                      >  2:276791/1‑81 (MQ=255)                                   cTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGt                                    <  2:276748/81‑1 (MQ=255)                                       tgatgGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATc                                 <  1:276749/80‑1 (MQ=255)                                        gatgGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCaa                               >  1:276790/1‑81 (MQ=255)                                               ctatctatATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCg                        <  1:276750/81‑1 (MQ=255)                                                  tctatATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCga                       <  2:276751/79‑1 (MQ=255)                                                      tatTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCgaaga                    <  2:276753/78‑1 (MQ=255)                                                      tatTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCgaag                     <  1:276752/77‑1 (MQ=255)                                                          gCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTa              <  1:276754/80‑1 (MQ=255)                                                           cGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTAc             >  1:276775/1‑80 (MQ=255)                                                            ggACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTAc             >  2:276798/1‑79 (MQ=255)                                                               cGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGc        <  2:276755/81‑1 (MQ=255)                                                                gCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCg       >  1:276809/1‑81 (MQ=255)                                                                 cTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCgg      <  1:276756/81‑1 (MQ=255)                                                                  tGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGt     >  1:276799/1‑81 (MQ=255)                                                                   gCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTg    >  2:276794/1‑81 (MQ=255)                                                                    cGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGc   >  2:276803/1‑81 (MQ=255)                                                                     gttgttCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCt  >  1:276812/1‑81 (MQ=255)                                                                             |                                                                         CCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCT  >  NC_000913/1512170‑1512318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑