Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,520,982	A→G	I695T (ATT→ACT)	yncD ←	putative TonB‑dependent outer membrane receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,520,982	0	A	G	100.0%	71.7 / NA	20	I695T (ATT→ACT)	yncD	putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/9);  total (11/9)

TTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGA  >  NC_000913/1520904‑1521054                                                                               |                                                                         ttATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAgta                                                                        >  2:278292/1‑81 (MQ=255) ttATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAgta                                                                        >  1:278301/1‑81 (MQ=255)    taCCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTc                                                                     <  2:278269/81‑1 (MQ=255)        aGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGc                                                                 <  2:278270/81‑1 (MQ=255)          cAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCg                                                               <  2:278272/81‑1 (MQ=255)          cAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCg                                                               <  2:278271/81‑1 (MQ=255)            aTACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCGa                                                              >  1:278291/1‑80 (MQ=255)                 aTCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCGACACCa                                                         >  1:278293/1‑80 (MQ=255)                       aaaGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCGACACCATAATTTc                                                  >  1:278285/1‑81 (MQ=255)                            aaaTGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCg                                             >  2:278294/1‑81 (MQ=255)                             aaTGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCgg                                            <  1:278273/81‑1 (MQ=255)                                 cccGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCg                                         <  1:278274/80‑1 (MQ=255)                                   cGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCGAAg                                      >  2:278295/1‑81 (MQ=255)                                           aCCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTaa                              >  2:278300/1‑81 (MQ=255)                                              gggCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAatat                           >  2:278302/1‑81 (MQ=255)                                                   tCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGccc                      <  2:278275/81‑1 (MQ=255)                                                               cTCAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCAtt          <  1:278276/81‑1 (MQ=255)                                                                 cAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGa        >  2:278299/1‑81 (MQ=255)                                                                 cAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGa        >  2:278305/1‑81 (MQ=255)                                                                   aaTCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGAc       >  2:278297/1‑80 (MQ=255)                                                                         cACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAAt    <  1:278277/77‑1 (MQ=255)                                                                          aCGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGa  >  1:278310/1‑78 (MQ=255)                                                                               |                                                                         TTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGA  >  NC_000913/1520904‑1521054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑