Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	104,426	C→T	L149F (CTT→TTT)	ftsA →	cell division protein FtsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	104,426	0	C	T	100.0%	154.3 / NA	40	L149F (CTT→TTT)	ftsA	cell division protein FtsA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/17);  total (23/17)

GATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCAAAGC  >  NC_000913/104348‑104502                                                                               |                                                                             gATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGAttt                                                                            <  1:21217/81‑1 (MQ=255) gATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGAttt                                                                            >  1:21279/1‑81 (MQ=255)    gAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCg                                                                         >  2:21256/1‑81 (MQ=255)    gAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCg                                                                         >  2:21254/1‑81 (MQ=255)    gAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCg                                                                         >  1:21265/1‑81 (MQ=255)     aGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCgg                                                                        >  1:21263/1‑81 (MQ=255)     aGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCgg                                                                        <  2:21218/81‑1 (MQ=255)       cATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGc                                                                      >  1:21239/1‑81 (MQ=255)        aTCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCg                                                                     >  1:21267/1‑81 (MQ=255)         tCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGt                                                                    <  1:21219/81‑1 (MQ=255)             gtgCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCg                                                                 >  2:21286/1‑80 (MQ=255)              tgctgcATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCgg                                                                <  1:21220/80‑1 (MQ=255)              tgctgcATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCgg                                                                <  1:21221/80‑1 (MQ=255)               gctgcATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGAt                                                              >  1:21282/1‑81 (MQ=255)                ctgcATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATg                                                             <  2:21222/81‑1 (MQ=255)                        gATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCaa                                                      >  2:21271/1‑80 (MQ=255)                          tCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAg                                                   <  1:21223/81‑1 (MQ=255)                                aaGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCAcc                                             >  2:21278/1‑81 (MQ=255)                                 agagTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCt                                            >  2:21291/1‑81 (MQ=255)                                   agTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGa                                          >  1:21285/1‑81 (MQ=255)                                      aTGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca                                       >  2:21268/1‑81 (MQ=255)                                       tGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTGTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATcac                                      <  2:21224/81‑1 (MQ=255)                                        gCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATcaca                                     >  1:21287/1‑81 (MQ=255)                                          gATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATg                                   <  2:21225/81‑1 (MQ=255)                                             tGACTATCAGGAAGGGATCAAGAATCCGGTATGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTc                                 <  2:21226/80‑1 (MQ=255)                                              gACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTca                                >  2:21274/1‑80 (MQ=255)                                               aCTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTcac                               <  2:21227/80‑1 (MQ=255)                                                  aTCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAAc                            >  1:21283/1‑80 (MQ=255)                                                     aGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGata                        >  2:21280/1‑81 (MQ=255)                                                     aGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGat                         <  1:21228/80‑1 (MQ=255)                                                      ggAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGatat                       <  2:21229/81‑1 (MQ=255)                                                           ggATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGa                  <  2:21230/81‑1 (MQ=255)                                                                aaGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaaa              >  2:21289/1‑80 (MQ=255)                                                                aaGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaaa              >  1:21301/1‑80 (MQ=255)                                                                 aGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACa            <  1:21231/81‑1 (MQ=255)                                                                  gAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACAt           >  2:21258/1‑81 (MQ=255)                                                                  gAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACAt           <  1:21232/81‑1 (MQ=255)                                                                   aaTCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATc          <  2:21233/81‑1 (MQ=255)                                                                     tCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGt        >  2:21311/1‑81 (MQ=255)                                                                         gTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCa      >  1:21277/1‑79 (MQ=255)                                                                         gTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCa      >  1:21310/1‑79 (MQ=255)                                                                            ggATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCAAAGc  <  2:21234/80‑1 (MQ=255)                                                                               |                                                                             GATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCAAAGC  >  NC_000913/104348‑104502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑