Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,607,828	C→T	G161G (GGC→GGT)	tam →	trans‑aconitate 2‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,607,828	0	C	T	100.0%	125.4 / NA	33	G161G (GGC→GGT)	tam	trans‑aconitate 2‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/16);  total (17/16)

GGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCAC  >  NC_000913/1607750‑1607900                                                                               |                                                                         ggAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGgtgt                                                                        >  2:292287/1‑81 (MQ=255)  gAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGtt                                                                       <  2:292257/81‑1 (MQ=255)    gCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCa                                                                     <  1:292258/81‑1 (MQ=255)    gCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCa                                                                     >  2:292289/1‑81 (MQ=255)       gACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGc                                                                  >  1:292295/1‑81 (MQ=255)               tgCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTtactac                                                           <  1:292260/80‑1 (MQ=255)               tgCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTtactac                                                           <  2:292259/80‑1 (MQ=255)                    aTGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATAtt                                                     <  1:292261/81‑1 (MQ=255)                      gcgcGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATAtttt                                                   >  2:292303/1‑81 (MQ=255)                            aGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGa                                             <  1:292262/81‑1 (MQ=255)                                gCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGc                                          <  1:292263/80‑1 (MQ=255)                                     ggAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGAtg                                    >  2:292300/1‑81 (MQ=255)                                     ggAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGAt                                     <  1:292264/80‑1 (MQ=255)                                        aCAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGAtgt                                   >  2:292298/1‑79 (MQ=255)                                        aCAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGa                                 <  2:292265/81‑1 (MQ=255)                                          aaaaCTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAgg                               >  1:292296/1‑81 (MQ=255)                                           aaaCTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGt                              <  1:292266/81‑1 (MQ=255)                                            aaCTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTc                             >  2:292315/1‑81 (MQ=255)                                             aCTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCg                            <  1:292267/81‑1 (MQ=255)                                              cTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGa                           >  2:292293/1‑81 (MQ=255)                                                 cccGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGatat                        >  1:292297/1‑81 (MQ=255)                                                  ccGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATc                       >  2:292309/1‑81 (MQ=255)                                                    ggATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTg                     >  2:292301/1‑81 (MQ=255)                                                     gATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTgg                    >  1:292302/1‑81 (MQ=255)                                                          cgGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAac               <  2:292268/81‑1 (MQ=255)                                                            ggCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAacca             >  1:292330/1‑81 (MQ=255)                                                             gCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAaccac            >  1:292307/1‑81 (MQ=255)                                                              cGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAaccacc           >  2:292310/1‑81 (MQ=255)                                                              cGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAaccacc           <  2:292269/81‑1 (MQ=255)                                                               gTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACct          <  2:292270/81‑1 (MQ=255)                                                                  aGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACctac        >  2:292304/1‑80 (MQ=255)                                                                    ccGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTAt     <  1:292271/81‑1 (MQ=255)                                                                     cGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATc    <  2:292272/81‑1 (MQ=255)                                                                       ttGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCAc  >  1:292306/1‑81 (MQ=255)                                                                           ctggtGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCAc  >  2:292308/1‑77 (MQ=255)                                                                               |                                                                         GGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCAC  >  NC_000913/1607750‑1607900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑