Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,610,589	A→G	V31A (GTT→GCT)	uxaB ←	tagaturonate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,610,589	0	A	G	100.0%	101.2 / NA	29	V31A (GTT→GCT)	uxaB	tagaturonate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/19);  total (10/19)

TCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGC  >  NC_000913/1610517‑1610661                                                                         |                                                                         tCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGgcg                                                                  >  1:292752/1‑81 (MQ=255) tCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGgc                                                                   <  1:292718/80‑1 (MQ=255)   aaTCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGgcgc                                                                 <  2:292719/80‑1 (MQ=255)     tCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCa                                                              <  2:292720/81‑1 (MQ=255)       ggACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAg                                                             <  2:292722/80‑1 (MQ=255)       ggACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAg                                                             <  2:292721/80‑1 (MQ=255)               aacGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTAcc                                                    >  1:292760/1‑81 (MQ=255)                acGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCt                                                   <  1:292723/81‑1 (MQ=255)                acGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCt                                                   <  2:292724/81‑1 (MQ=255)                 cGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCtt                                                  <  1:292725/81‑1 (MQ=255)                  gACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCtt                                                  >  2:292763/1‑80 (MQ=255)                   aCCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCa                                                <  2:292726/81‑1 (MQ=255)                      aCGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCa                                             <  1:292727/81‑1 (MQ=255)                           aGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACt                                         >  1:292753/1‑80 (MQ=255)                           aGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACt                                         >  2:292750/1‑80 (MQ=255)                                 cAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGa                                   <  2:292728/80‑1 (MQ=255)                                   gATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATAc                                <  2:292729/81‑1 (MQ=255)                                     tCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGt                              >  2:292783/1‑81 (MQ=255)                                        gtgtGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCt                           >  2:292761/1‑81 (MQ=255)                                             cTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGAt                       <  1:292730/80‑1 (MQ=255)                                               cATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACt                    >  1:292762/1‑81 (MQ=255)                                                aTTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATAc                     <  2:292731/79‑1 (MQ=255)                                                 ttCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACtgt                  <  1:292732/81‑1 (MQ=255)                                                    aGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGc                <  2:292733/80‑1 (MQ=255)                                                         aTCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACCGAATGATACGTTCTGGATACTGTGCACCgg           >  2:292758/1‑80 (MQ=255)                                                            gATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGaaa       <  1:292735/81‑1 (MQ=255)                                                            gATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGaaa       <  2:292734/81‑1 (MQ=255)                                                            gATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGaa        >  1:292766/1‑80 (MQ=255)                                                                  ccAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATcgc  <  1:292736/80‑1 (MQ=255)                                                                         |                                                                         TCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGC  >  NC_000913/1610517‑1610661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑