Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,658,706	G→C	G213A (GGC→GCC)	ynfE →	putative selenate reductase YnfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,658,706	0	G	C	100.0%	106.0 / NA	29	G213A (GGC→GCC)	ynfE	putative selenate reductase YnfE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (15/14);  total (15/14)

GTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGGCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGCG  >  NC_000913/1658628‑1658783                                                                               |                                                                              gTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCa                                                                             <  1:299759/81‑1 (MQ=255)    cACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAAc                                                                           <  1:299760/80‑1 (MQ=255)             aGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACg                                                                  >  1:299783/1‑80 (MQ=255)               tATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGat                                                               <  1:299761/81‑1 (MQ=255)                aTGGCTCCTACAGCACTGCGCAAAATTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGata                                                              >  2:299793/1‑81 (MQ=255)                            gCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCa                                                  <  1:299762/81‑1 (MQ=255)                             cACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCaa                                                 <  1:299763/81‑1 (MQ=255)                               cTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGc                                               <  2:299764/81‑1 (MQ=255)                                 gcgcAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTg                                             >  1:299792/1‑81 (MQ=255)                                 gcgcAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTg                                             >  2:299800/1‑81 (MQ=255)                                  cgcAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTg                                             <  1:299765/80‑1 (MQ=255)                                     aaaTTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCg                                         >  2:299789/1‑81 (MQ=255)                                          tCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATg                                    >  2:299788/1‑81 (MQ=255)                                            cTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGtt                                  <  1:299767/81‑1 (MQ=255)                                            cTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGtt                                  <  1:299766/81‑1 (MQ=255)                                                gCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTgg                               >  2:299791/1‑80 (MQ=255)                                                      cccTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTaacaac                        >  1:299796/1‑81 (MQ=255)                                                       ccTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAAcc                       <  2:299768/81‑1 (MQ=255)                                                        cTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAAccc                      >  1:299795/1‑81 (MQ=255)                                                           cacCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCg                     <  2:299769/79‑1 (MQ=255)                                                            acCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCgg                    >  2:299804/1‑79 (MQ=255)                                                             ccTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAg                 <  2:299770/81‑1 (MQ=255)                                                                  ggCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAAcc            >  2:299797/1‑81 (MQ=255)                                                                    cAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCg          >  1:299801/1‑81 (MQ=255)                                                                     aGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCg          >  1:299798/1‑80 (MQ=255)                                                                        aaTGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCAt       >  1:299799/1‑80 (MQ=255)                                                                           gatgCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGc   >  2:299802/1‑81 (MQ=255)                                                                            atgCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGc   <  1:766774/80‑1 (MQ=255)                                                                            atgCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGCg  <  1:299771/81‑1 (MQ=255)                                                                               |                                                                              GTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGGCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGCG  >  NC_000913/1658628‑1658783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑