Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,680,663	C→T	P369P (CCC→CCT)	ydgI →	putative arginine:ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,680,663	0	C	T	100.0%	138.8 / NA	36	P369P (CCC→CCT)	ydgI	putative arginine:ornithine antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/15);  total (21/15)

GTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTG  >  NC_000913/1680587‑1680733                                                                             |                                                                       gTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTAtt                                                                    >  2:303486/1‑81 (MQ=255)  tCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTAtt                                                                    <  1:303460/80‑1 (MQ=255)   cTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTc                                                                  >  2:303492/1‑81 (MQ=255)   cTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTc                                                                  >  1:303501/1‑81 (MQ=255)      gTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCt                                                                <  2:303461/80‑1 (MQ=255)      gTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTg                                                               <  1:303463/81‑1 (MQ=255)      gTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTg                                                               <  2:303462/81‑1 (MQ=255)        aCTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGt                                                             >  2:303490/1‑81 (MQ=255)            aTCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGgc                                                         <  2:303464/81‑1 (MQ=255)              cTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGgcgc                                                       >  2:303502/1‑81 (MQ=255)                ggCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGt                                                     >  2:303497/1‑81 (MQ=255)                ggCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGt                                                     >  2:303488/1‑81 (MQ=255)                 gCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGtt                                                    >  2:303504/1‑81 (MQ=255)                  cTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTc                                                   >  1:303514/1‑81 (MQ=255)                     aCCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCt                                                  <  2:303465/79‑1 (MQ=255)                      ccGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGc                                               >  1:303499/1‑81 (MQ=255)                        ggTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCt                                              >  1:303508/1‑80 (MQ=255)                        ggTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCt                                              >  1:303489/1‑80 (MQ=255)                        ggTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGCGCCTTATTTCCTGGTCGGCGCGTTCTTGCt                                              >  2:303494/1‑80 (MQ=255)                            ccGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGaaaa                                         >  2:303495/1‑81 (MQ=255)                             cGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGaaaa                                         <  1:303466/80‑1 (MQ=255)                              gATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATc                                       >  1:303496/1‑81 (MQ=255)                                  acaacaCGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCaa                                   >  2:303516/1‑81 (MQ=255)                                  acaacaCGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCa                                    <  2:303467/80‑1 (MQ=255)                                     acacGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAAccc                                >  2:303500/1‑81 (MQ=255)                                      cacGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCg                               <  2:303468/81‑1 (MQ=255)                                       acGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGt                              >  1:303507/1‑81 (MQ=255)                                        cGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTc                             <  2:303469/81‑1 (MQ=255)                                            gCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCAc                          <  1:303471/80‑1 (MQ=255)                                            gCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCAc                          <  1:303470/80‑1 (MQ=255)                                            gCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACt                         >  1:303511/1‑81 (MQ=255)                                                      gCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGc                <  2:303472/80‑1 (MQ=255)                                                        cTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCgg              >  2:303491/1‑80 (MQ=255)                                                        cTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGt             <  1:303473/81‑1 (MQ=255)                                                           aGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAg           <  1:303474/80‑1 (MQ=255)                                                                   ttCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTg  >  1:303510/1‑81 (MQ=255)                                                                             |                                                                       GTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTG  >  NC_000913/1680587‑1680733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑