Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,774,321	T→C	V178A (GTC→GCC)	ydiB →	shikimate dehydrogenase/quinate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,774,321	0	T	C	100.0%	80.2 / NA	23	V178A (GTC→GCC)	ydiB	shikimate dehydrogenase/quinate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/14);  total (9/14)

AACTCTTTAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATG  >  NC_000913/1774243‑1774395                                                                               |                                                                           aaCTCTTTAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTgccg                                                                          >  1:319147/1‑81 (MQ=255)   ctctTTAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTgccgtc                                                                        <  2:319123/81‑1 (MQ=255)     ctTTAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCAc                                                                      >  1:319165/1‑81 (MQ=255)        tAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCAc                                                                      <  2:319124/78‑1 (MQ=255)         aaCCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGt                                                                   >  2:319157/1‑80 (MQ=255)         aaCCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGt                                                                   >  1:319153/1‑80 (MQ=255)          aCCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTc                                                                  <  2:319125/80‑1 (MQ=255)                  gATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGAtctc                                                         <  2:319126/81‑1 (MQ=255)                      aGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGcc                                                      <  2:319127/80‑1 (MQ=255)                      aGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCg                                                     <  2:717069/81‑1 (MQ=255)                           ttcGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAg                                                >  2:319161/1‑81 (MQ=255)                                 aaaGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTGACCGATCTCGCCGATCAGCAAGcc                                          >  2:319163/1‑81 (MQ=255)                                  aaGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCt                                         >  1:319166/1‑81 (MQ=255)                                    gCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCttt                                       <  2:319128/81‑1 (MQ=255)                                                gcgcAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTg                           <  2:319129/81‑1 (MQ=255)                                                gcgcAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTg                           <  1:319130/81‑1 (MQ=255)                                                  gcAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGc                         >  2:319159/1‑81 (MQ=255)                                                      cGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTcc                     <  1:319131/81‑1 (MQ=255)                                                       gggTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTccg                    <  2:319132/81‑1 (MQ=255)                                                           tAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGa                >  1:319164/1‑81 (MQ=255)                                                             aTGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGAc               <  1:319134/80‑1 (MQ=255)                                                             aTGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGAc               <  1:319133/80‑1 (MQ=255)                                                              tGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACAt             <  1:319135/81‑1 (MQ=255)                                                                   acacCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTaa        >  1:319158/1‑81 (MQ=255)                                                                         aTTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATg  <  2:319136/81‑1 (MQ=255)                                                                               |                                                                           AACTCTTTAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATG  >  NC_000913/1774243‑1774395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑