Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,796,866	T→C	Y360C (TAT→TGT)	pheT ←	phenylalanine‑‑tRNA ligase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,796,866	0	T	C	100.0%	126.9 / NA	34	Y360C (TAT→TGT)	pheT	phenylalanine‑‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/18);  total (16/18)

CGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACG  >  NC_000913/1796792‑1796938                                                                           |                                                                         cGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGt                                                                    <  1:322936/81‑1 (MQ=255) cGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGt                                                                    >  1:322974/1‑81 (MQ=255)     gATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTgaga                                                                >  1:322996/1‑81 (MQ=255)      aTGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGAc                                                               >  1:322998/1‑81 (MQ=255)        gTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACg                                                              >  1:322985/1‑80 (MQ=255)           gATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATc                                                          >  1:322976/1‑81 (MQ=255)            aTCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCg                                                         <  1:322937/81‑1 (MQ=255)            aTCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCg                                                         >  1:322986/1‑81 (MQ=255)               agcagACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTa                                                      >  2:322979/1‑81 (MQ=255)                  agACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATg                                                    >  2:322983/1‑80 (MQ=255)                      gggTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGc                                               <  1:322938/81‑1 (MQ=255)                      gggTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGc                                               >  2:322981/1‑81 (MQ=255)                        gTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCa                                             <  2:322939/81‑1 (MQ=255)                           gCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATg                                           <  1:322940/80‑1 (MQ=255)                             aCGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATgac                                         >  1:322984/1‑80 (MQ=255)                                  ccATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAg                                   >  2:322997/1‑81 (MQ=255)                                          ttGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCg                           >  1:322991/1‑81 (MQ=255)                                           tgtgCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAAGCCATGACGACGAGCACGAcc                            <  2:322941/79‑1 (MQ=255)                                             tgctgcAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGt                         <  1:322942/80‑1 (MQ=255)                                             tgctgcAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGt                         <  2:322943/80‑1 (MQ=255)                                                  cAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATa                     <  2:322944/79‑1 (MQ=255)                                                   aGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGAc                  <  1:322945/81‑1 (MQ=255)                                                     tGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAg                >  1:323013/1‑81 (MQ=255)                                                     tGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAg                <  2:322946/81‑1 (MQ=255)                                                       ccGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCg              >  1:322994/1‑81 (MQ=255)                                                        cGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCgg             <  1:322947/81‑1 (MQ=255)                                                           aTCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGc           >  1:323000/1‑80 (MQ=255)                                                            tCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTa         <  1:322948/81‑1 (MQ=255)                                                              aaCGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTa         >  2:322966/1‑79 (MQ=255)                                                               aCGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAg      <  1:322950/81‑1 (MQ=255)                                                               aCGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAg      <  2:322949/81‑1 (MQ=255)                                                                  ccACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAAc   <  1:322951/81‑1 (MQ=255)                                                                  ccACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAAc   <  1:322952/81‑1 (MQ=255)                                                                   cACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAAcg  <  1:322953/81‑1 (MQ=255)                                                                           |                                                                         CGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACG  >  NC_000913/1796792‑1796938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑