Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,830,943	T→C	Y347C (TAC→TGC)	astC ←	succinylornithine transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,830,943	0	T	C	100.0%	87.3 / NA	24	Y347C (TAC→TGC)	astC	succinylornithine transaminase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/16);  total (8/16)

CCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAAT  >  NC_000913/1830866‑1831019                                                                              |                                                                             ccacGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAAt                                                                           <  2:328588/81‑1 (MQ=255)  cacGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATc                                                                          <  2:328589/81‑1 (MQ=255)   acGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCg                                                                         <  2:328590/81‑1 (MQ=255)     gTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCgg                                                                        >  1:328621/1‑80 (MQ=255)         ccACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTc                                                                   <  1:328591/81‑1 (MQ=255)            cccGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGt                                                                <  2:328592/81‑1 (MQ=255)                 aaTCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTAcaca                                                           <  2:328593/81‑1 (MQ=255)                    cAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGc                                                         >  1:328627/1‑80 (MQ=255)                     aGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGcc                                                        <  1:328594/80‑1 (MQ=255)                     aGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGcc                                                        >  2:328622/1‑80 (MQ=255)                      gTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCaa                                                      <  1:328595/81‑1 (MQ=255)                        aCCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAAt                                                     >  1:328624/1‑80 (MQ=255)                         ccATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATc                                                    <  2:328596/80‑1 (MQ=255)                                           ccgcTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGaa                                 >  1:328626/1‑81 (MQ=255)                                                  cTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACt                          <  2:328598/81‑1 (MQ=255)                                                      gagaTCTGTCTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCTTTGaa                       >  2:328629/1‑80 (MQ=255)                                                        gaTCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACaa                    <  2:328599/81‑1 (MQ=255)                                                              tttCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAAc                  <  1:328600/77‑1 (MQ=255)                                                                tCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAg             <  2:328601/80‑1 (MQ=255)                                                                     tGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGa       >  2:328628/1‑81 (MQ=255)                                                                      gCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTg        <  2:328602/79‑1 (MQ=255)                                                                        ccGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTa    <  1:328603/81‑1 (MQ=255)                                                                         cGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTaa   >  2:328631/1‑81 (MQ=255)                                                                          ggCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAAt  <  2:328604/81‑1 (MQ=255)                                                                              |                                                                             CCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAAT  >  NC_000913/1830866‑1831019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑