Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,832,618	G→C	G64A (GGC→GCC)	xthA →	exodeoxyribonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,832,618	0	G	C	100.0%	140.8 / NA	38	G64A (GGC→GCC)	xthA	exodeoxyribonuclease III
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (19/19);  total (19/19)

CGATATGTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGC  >  NC_000913/1832544‑1832692                                                                           |                                                                           cGATATGTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTAtgccgtagc                                                                      <  2:328894/81‑1 (MQ=255)   atatGTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGgcgc                                                                    >  1:328932/1‑81 (MQ=255)     atGTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCgctg                                                                  >  1:328934/1‑81 (MQ=255)       gTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCgctgct                                                                >  2:328953/1‑81 (MQ=255)        tttCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCgctgct                                                                <  1:328895/80‑1 (MQ=255)        tttCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCgctgct                                                                <  2:328896/80‑1 (MQ=255)        tttCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCgctgct                                                                >  1:328929/1‑80 (MQ=255)            cGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCa                                                           <  2:328897/81‑1 (MQ=255)              cccGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCa                                                           >  2:328958/3‑79 (MQ=255)              cTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCaaa                                                         <  2:328898/81‑1 (MQ=255)                cGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCaaa                                                         >  1:328931/1‑79 (MQ=255)                cGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCaaa                                                         >  2:328944/1‑79 (MQ=255)                 gAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAag                                                        <  2:328899/79‑1 (MQ=255)                  aaGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAgaga                                                     >  1:328949/1‑81 (MQ=255)                   agagGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGAc                                                    >  2:328940/1‑81 (MQ=255)                   agagGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGAc                                                    <  1:328900/81‑1 (MQ=255)                    gagGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACg                                                   <  1:328901/81‑1 (MQ=255)                      ggTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGcc                                                 <  2:328902/81‑1 (MQ=255)                      ggTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGcc                                                 <  1:328903/81‑1 (MQ=255)                            gAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGc                                           >  1:328936/1‑81 (MQ=255)                             aaGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGcc                                          >  1:328951/1‑81 (MQ=255)                              aGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCg                                         <  1:328904/81‑1 (MQ=255)                                   ggCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGt                                    >  1:328938/1‑81 (MQ=255)                                    gCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTc                                   <  1:328905/81‑1 (MQ=255)                                         aaCGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAGAGAGACGCCGATTGCCGTGCGTCGCGGc                              >  2:328943/1‑81 (MQ=255)                                          aCGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCt                             >  2:328960/1‑81 (MQ=255)                                               ttttATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTccc                        <  1:328906/81‑1 (MQ=255)                                                 ttATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCg                       <  1:328907/80‑1 (MQ=255)                                                    tCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTg                    <  2:328908/80‑1 (MQ=255)                                                        gggCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTgacgac               >  1:328945/1‑81 (MQ=255)                                                        gggCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTgacgac               >  2:328957/1‑81 (MQ=255)                                                        gggCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTgacgac               <  1:328909/81‑1 (MQ=255)                                                        gggCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTgacgac               <  1:328910/81‑1 (MQ=255)                                                         ggCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGacgacg              <  1:328911/81‑1 (MQ=255)                                                           cAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGacgacg              >  2:328964/1‑79 (MQ=255)                                                             gAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAaga          <  2:328912/81‑1 (MQ=255)                                                                aGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGgc       >  2:328965/1‑81 (MQ=255)                                                                    cATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAg   <  2:328913/81‑1 (MQ=255)                                                                     aTTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAgc  >  1:328952/1‑81 (MQ=255)                                                                           |                                                                           CGATATGTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGC  >  NC_000913/1832544‑1832692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑