Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,928,777	A→G	D21D (GAT→GAC)	ptrB ←	oligopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,928,777	0	A	G	100.0%	120.6 / NA	33	D21D (GAT→GAC)	ptrB	oligopeptidase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/19);  total (14/19)

ACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCT  >  NC_000913/1928699‑1928854                                                                               |                                                                              aCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTc                                                                             <  1:345202/81‑1 (MQ=255)   cGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGa                                                                           >  1:345235/1‑81 (MQ=255)     tAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATg                                                                         >  2:345244/1‑81 (MQ=255)        cTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATgcgc                                                                      <  1:345203/81‑1 (MQ=255)         tATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGcgcg                                                                     >  1:345246/1‑81 (MQ=255)              tCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATc                                                                 <  2:345204/80‑1 (MQ=255)              tCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCg                                                                >  2:345239/1‑81 (MQ=255)                  gttgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCAt                                                            >  1:345238/1‑81 (MQ=255)                            gTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCAt                                                  <  2:345205/81‑1 (MQ=255)                             tCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATg                                                 <  2:345206/81‑1 (MQ=255)                               cAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATgg                                                <  2:345207/80‑1 (MQ=255)                               cAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGc                                               >  2:345240/1‑81 (MQ=255)                                   aCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTgg                                           <  2:345208/81‑1 (MQ=255)                                      tCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTgggg                                         >  1:345248/1‑80 (MQ=255)                                        tGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAAt                                      >  2:345254/1‑81 (MQ=255)                                         ggCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATg                                     >  1:345251/1‑81 (MQ=255)                                          gCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAAt                                      <  2:345209/79‑1 (MQ=255)                                                  cgcgTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCt                            <  2:345210/81‑1 (MQ=255)                                                   gcgTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCtt                           <  2:345211/81‑1 (MQ=255)                                                      tATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTg                        >  2:345255/1‑81 (MQ=255)                                                        tCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTgg                       <  2:345212/80‑1 (MQ=255)                                                        tCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGt                      <  2:345213/81‑1 (MQ=255)                                                           tCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGc                   <  2:345214/81‑1 (MQ=255)                                                             ccGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCAt                 <  1:345215/81‑1 (MQ=255)                                                              cGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATg                >  1:345247/1‑81 (MQ=255)                                                               gCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGt               >  1:345245/1‑81 (MQ=255)                                                                   ccAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTAtt           <  1:345216/81‑1 (MQ=255)                                                                         gtaATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttctt     <  2:345217/81‑1 (MQ=255)                                                                         gtaATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttct      <  2:345218/80‑1 (MQ=255)                                                                           aaTTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttctttc   >  1:345252/1‑81 (MQ=255)                                                                           aaTTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttctttc   >  1:345250/1‑81 (MQ=255)                                                                            aTTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttctttct  <  1:345220/81‑1 (MQ=255)                                                                            aTTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttctttct  <  2:345219/81‑1 (MQ=255)                                                                               |                                                                              ACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCT  >  NC_000913/1928699‑1928854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑