Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,995,681	T→C	Y9Y (TAT→TAC)	yecU →	protein YecU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,995,681	0	T	C	100.0%	32.9 / NA	10	Y9Y (TAT→TAC)	yecU	protein YecU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/7);  total (3/7)

CGCTGAATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTAT  >  NC_000913/1995604‑1995752                                                                              |                                                                        cGCTGAATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACAtt                                                                      <  2:365709/81‑1 (MQ=255)  gCTGAATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACAtt                                                                      >  1:357156/1‑80 (MQ=255)        tatGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTAt                                                               <  2:357143/81‑1 (MQ=255)                attaattaACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAg                                                        >  1:357155/1‑80 (MQ=255)                     ttaaCCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGc                                                  >  2:357154/1‑81 (MQ=255)                       aaCCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGcc                                                 <  1:357145/80‑1 (MQ=255)                       aaCCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGcc                                                 <  2:357144/80‑1 (MQ=255)                                      ggCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCaaaa                                 <  2:357146/81‑1 (MQ=255)                                                aTTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACt                       <  1:357147/81‑1 (MQ=255)                                                                      ggTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTAt  <  2:357148/80‑1 (MQ=255)                                                                              |                                                                        CGCTGAATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTAT  >  NC_000913/1995604‑1995752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑