Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,995,748	C→G	L32V (CTT→GTT)	yecU →	protein YecU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,995,748	0	C	G	100.0%	31.9 / NA	10	L32V (CTT→GTT)	yecU	protein YecU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (7/3);  total (7/3)

GGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCA  >  NC_000913/1995673‑1995799                                                                            |                                                    ggTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTAt                                                 <  2:357148/80‑1 (MQ=255)       tacatTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATAc                                          >  2:357162/4‑81 (MQ=255)              aCGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTccgccg                                   >  2:357163/1‑81 (MQ=255)                   ttttATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTa                              <  2:357149/81‑1 (MQ=255)                            aCTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACAttttt                      >  2:357161/1‑80 (MQ=255)                                   atatCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCa               >  2:357159/1‑80 (MQ=255)                                        aCGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAAtttt         >  1:357170/1‑81 (MQ=255)                                          gCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCa       >  2:357158/1‑81 (MQ=255)                                              aaaaaaCAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTg    >  1:357166/1‑80 (MQ=255)                                               aaaaaCAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCa  <  2:357150/81‑1 (MQ=255)                                                                            |                                                    GGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCA  >  NC_000913/1995673‑1995799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑