Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,015,409	A→G	E177G (GAA→GGA)	fliG →	flagellar motor switch protein FliG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,015,409	0	A	G	100.0%	124.4 / NA	34	E177G (GAA→GGA)	fliG	flagellar motor switch protein FliG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/16);  total (18/16)

TCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAAT  >  NC_000913/2015335‑2015484                                                                           |                                                                            tCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACt                                                                       <  1:360297/81‑1 (MQ=255) tCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACt                                                                       >  1:360341/1‑81 (MQ=255)  cTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACt                                                                       >  2:360350/1‑80 (MQ=255)    gcgcCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGaa                                                                    >  1:360345/1‑81 (MQ=255)      gcCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATg                                                                  <  2:360299/81‑1 (MQ=255)        cACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGc                                                                >  2:360343/1‑81 (MQ=255)          cgacgTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCtt                                                              <  1:360300/81‑1 (MQ=255)             cgTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCt                                                           >  2:360358/1‑81 (MQ=255)               tGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTc                                                          >  1:360346/1‑80 (MQ=255)                 aTGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGAc                                                       >  2:360357/1‑81 (MQ=255)                 aTGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGAc                                                       >  1:360352/1‑81 (MQ=255)                   gTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACgg                                                     >  2:360349/1‑81 (MQ=255)                    ttGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGt                                                    >  1:360354/1‑81 (MQ=255)                      gCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTc                                                   <  1:360301/80‑1 (MQ=255)                             gCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAAtct                                            <  1:360302/80‑1 (MQ=255)                             gCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAAtct                                            >  2:360326/1‑80 (MQ=255)                                     tGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCgcag                                   >  1:360344/1‑81 (MQ=255)                                      ggcggcGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCgcag                                   <  2:360303/80‑1 (MQ=255)                                           cgTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAAt                             <  2:360304/81‑1 (MQ=255)                                            gTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATg                            >  1:360355/1‑81 (MQ=255)                                            gTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATg                            >  2:360356/1‑81 (MQ=255)                                             tGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATgg                           <  1:360305/81‑1 (MQ=255)                                               cagccagcCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGggc                         <  1:360306/81‑1 (MQ=255)                                                agccagccGCGCTGGCGGGGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGggcg                        <  2:360307/81‑1 (MQ=255)                                                  ccagccGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGggcggc                      <  1:360309/81‑1 (MQ=255)                                                  ccagccGCGCTGGAGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGggcggc                      <  1:360308/81‑1 (MQ=255)                                                    agccGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGt                    >  2:360361/1‑81 (MQ=255)                                                         cgcTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGaa               <  2:360310/81‑1 (MQ=255)                                                              gCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCa          <  2:360311/81‑1 (MQ=255)                                                                 gAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGcc       <  1:360312/81‑1 (MQ=255)                                                                      gACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAAt  >  2:360366/1‑81 (MQ=255)                                                                      gACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAAt  >  1:360362/1‑81 (MQ=255)                                                                       aCCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGaaa   <  2:360313/79‑1 (MQ=255)                                                                       aCCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAAt  >  1:360376/1‑80 (MQ=255)                                                                           |                                                                            TCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAAT  >  NC_000913/2015335‑2015484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑