Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,113,482	T→C	K282K (AAA→AAG)	galF ←	UTP:glucose‑1‑phosphate uridylyltransferase, low activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,113,482	0	T	C	100.0%	93.2 / NA	26	K282K (AAA→AAG)	galF	UTP:glucose‑1‑phosphate uridylyltransferase, low activity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/15);  total (11/15)

TCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGG  >  NC_000913/2113406‑2113558                                                                             |                                                                             tCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCcttccttc                                                                           <  1:376538/80‑1 (MQ=255)       gTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGc                                                                    >  1:376566/1‑81 (MQ=255)             tCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGc                                                              <  2:376539/81‑1 (MQ=255)                ggTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCa                                                            >  1:376574/1‑80 (MQ=255)                ggTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCAt                                                           <  1:470658/81‑1 (MQ=255)                   cAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACt                                                        >  2:376567/1‑81 (MQ=255)                    aGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACtt                                                       <  1:376540/81‑1 (MQ=255)                     gATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTc                                                      <  2:466696/81‑1 (MQ=255)                      aTTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTca                                                     <  1:376541/81‑1 (MQ=255)                             ttattCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGc                                              <  1:376542/81‑1 (MQ=255)                              tattCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGcc                                             >  1:376570/1‑81 (MQ=255)                               attCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCt                                            >  1:376571/1‑81 (MQ=255)                                 tCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGTCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTg                                           >  2:376562/1‑80 (MQ=255)                                  cGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGc                                          <  2:376543/80‑1 (MQ=255)                                        aCAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAg                                    <  1:376544/80‑1 (MQ=255)                                          aGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGcc                                  >  2:376583/1‑80 (MQ=255)                                             ttCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCAtt                              >  1:376576/1‑81 (MQ=255)                                               ctcAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCAtt                              <  1:376545/79‑1 (MQ=255)                                                  aaTACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCAttttttt                         >  2:376569/1‑81 (MQ=255)                                                       cTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGc                    <  1:376546/81‑1 (MQ=255)                                                          tACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAg                  <  2:376547/80‑1 (MQ=255)                                                           aCGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTc                >  2:376589/1‑81 (MQ=255)                                                            cGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTc                <  2:376548/80‑1 (MQ=255)                                                            cGGAACTTCACCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCg               >  1:376580/1‑81 (MQ=255)                                                              gAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTa             <  1:376549/81‑1 (MQ=255)                                                                    cGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGt        <  2:376550/80‑1 (MQ=255)                                                                       cccTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGcc    <  2:376551/81‑1 (MQ=255)                                                                         cttccttcAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCgg  <  1:376552/81‑1 (MQ=255)                                                                             |                                                                             TCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGG  >  NC_000913/2113406‑2113558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑