Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	191,485	A→G	E210G (GAA→GGA)	tsf →	protein chain elongation factor EF‑Ts

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	191,485	0	A	G	100.0%	111.7 / NA	30	E210G (GAA→GGA)	tsf	protein chain elongation factor EF‑Ts
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/14);  total (16/14)

GACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTT  >  NC_000913/191409‑191557                                                                             |                                                                         gACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATc                                                                      >  1:38441/1‑81 (MQ=255)   cGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCg                                                                     >  2:38442/1‑80 (MQ=255)               aGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGt                                                        >  1:38439/1‑81 (MQ=255)                   gTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGa                                                     <  1:38418/80‑1 (MQ=255)                     aGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAgg                                                  >  2:183979/1‑81 (MQ=255)                        aaaaGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCg                                               >  2:38452/1‑81 (MQ=255)                            gAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATg                                           <  1:38420/81‑1 (MQ=255)                            gAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATg                                           <  2:38419/81‑1 (MQ=255)                             aaTACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATg                                           >  2:38450/1‑80 (MQ=255)                             aaTACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATg                                           >  1:38466/1‑80 (MQ=255)                                aCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATgaag                                        >  1:38453/1‑80 (MQ=255)                                    ggTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAAtt                                   >  1:38445/1‑81 (MQ=255)                                        cAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCAcc                               <  1:38422/81‑1 (MQ=255)                                        cAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCAc                                <  1:38421/80‑1 (MQ=255)                                         aGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCg                              <  2:38423/81‑1 (MQ=255)                                              gATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGaa                         <  1:38424/81‑1 (MQ=255)                                              gATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGaa                         <  1:38425/81‑1 (MQ=255)                                                 atCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGtt                      >  1:38446/1‑81 (MQ=255)                                                 atCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGtt                      >  1:38447/1‑81 (MQ=255)                                                 atCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGtt                      >  1:38454/1‑81 (MQ=255)                                                      gATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTctct                 <  1:38426/81‑1 (MQ=255)                                                         gCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGAc              >  2:38457/1‑81 (MQ=255)                                                           aGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCg            >  1:38462/1‑81 (MQ=255)                                                                 gTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGc      <  1:38427/81‑1 (MQ=255)                                                                 gTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGc      >  1:38461/1‑81 (MQ=255)                                                                  tAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGcc     <  2:38428/81‑1 (MQ=255)                                                                   aaGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcg    >  1:38455/1‑81 (MQ=255)                                                                    aGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgt   <  2:38429/81‑1 (MQ=255)                                                                    aGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgt   <  1:38430/81‑1 (MQ=255)                                                                     gCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgtt  <  1:38431/81‑1 (MQ=255)                                                                             |                                                                         GACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTT  >  NC_000913/191409‑191557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑