Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,221,701	T→C	M97V (ATG→GTG)	bglX ←	beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,221,701	0	T	C	100.0%	89.5 / NA	25	M97V (ATG→GTG)	bglX	beta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/13);  total (12/13)

CGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGATC  >  NC_000913/2221627‑2221777                                                                           |                                                                             cGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCt                                                                         >  2:396291/1‑80 (MQ=255)   ggAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGAt                                                                      <  1:396241/81‑1 (MQ=255)                     cGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGatat                                                    <  1:396242/81‑1 (MQ=255)                      gTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATc                                                   <  1:396243/81‑1 (MQ=255)                      gTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATCCCACCACCTGATCCTGCATGGCGCGGATATc                                                   >  2:396290/1‑81 (MQ=255)                             aCGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACgg                                            <  1:396244/81‑1 (MQ=255)                               gtcgtAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGt                                          <  1:396245/81‑1 (MQ=255)                                   tAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACg                                      <  1:396246/81‑1 (MQ=255)                                   tAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACg                                      >  2:396280/1‑81 (MQ=255)                                     aGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGgt                                    <  1:396247/81‑1 (MQ=255)                                         aagaaaagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGtt                                 >  1:396283/1‑80 (MQ=255)                                          agaaaagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTg                                >  1:396281/1‑80 (MQ=255)                                          agaaaagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTg                                >  2:396295/1‑80 (MQ=255)                                            aaaagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTCGaa                              >  1:396279/1‑80 (MQ=255)                                               agagGAATTTTCAGGCGGCTTAAGTCCACCACCTGATCCTGCAGGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATc                          <  1:396248/81‑1 (MQ=255)                                                         tCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAAcc                 <  2:396249/80‑1 (MQ=255)                                                         tCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAAcc                 <  1:396250/80‑1 (MQ=255)                                                         tCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCt                >  1:396300/1‑81 (MQ=255)                                                         tCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCt                >  2:396278/1‑81 (MQ=255)                                                          cAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCt                >  2:396289/1‑80 (MQ=255)                                                             gcggcTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGAcc            <  2:396252/81‑1 (MQ=255)                                                             gcggcTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGAcc            <  1:396251/81‑1 (MQ=255)                                                                     aTTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGa    >  1:396285/1‑81 (MQ=255)                                                                     aTTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGa    <  2:396253/81‑1 (MQ=255)                                                                      ttCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGat   >  1:396284/1‑81 (MQ=255)                                                                        ccaccaCCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGatc  <  1:396254/80‑1 (MQ=255)                                                                           |                                                                             CGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGATC  >  NC_000913/2221627‑2221777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑