Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,260,779	T→C	K211R (AAA→AGA)	fruA ←	fructose‑specific PTS multiphosphoryl transfer protein FruA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,260,779	0	T	C	100.0%	122.3 / NA	34	K211R (AAA→AGA)	fruA	fructose‑specific PTS multiphosphoryl transfer protein FruA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/20);  total (14/20)

AACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCG  >  NC_000913/2260709‑2260856                                                                       |                                                                              aaCGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCgg                                                                     <  2:403147/81‑1 (MQ=255)    gCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTc                                                                  <  1:403148/81‑1 (MQ=255)        gTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATAc                                                              >  2:403190/1‑81 (MQ=255)         tCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACg                                                             <  2:403149/81‑1 (MQ=255)           aGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGc                                                           >  2:403200/1‑81 (MQ=255)             cAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGt                                                         <  2:403150/81‑1 (MQ=255)              aaGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGtt                                                        >  2:403191/1‑81 (MQ=255)                  gACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCtt                                                    >  1:403202/1‑81 (MQ=255)                   aCGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCtt                                                    <  1:403151/80‑1 (MQ=255)                   aCGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTc                                                   <  1:403152/81‑1 (MQ=255)                    cGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCa                                                  <  1:403153/81‑1 (MQ=255)                       tATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCa                                               <  1:403155/81‑1 (MQ=255)                       tATGCGCATGCACTCTCTTTCTTACTTTAAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCa                                               <  1:403154/81‑1 (MQ=255)                           cgcCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAAcc                                            <  1:403156/80‑1 (MQ=255)                           cgcCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAAcc                                            <  2:403157/80‑1 (MQ=255)                             ccTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCt                                         >  1:403199/1‑81 (MQ=255)                                gCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTa                                      >  2:403198/1‑81 (MQ=255)                                     ctctTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAg                                 >  1:403196/1‑81 (MQ=255)                                           cttACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCt                            <  2:403158/80‑1 (MQ=255)                                              aCTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTgcg                         <  2:403159/80‑1 (MQ=255)                                               cTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGcgcg                       <  1:403160/81‑1 (MQ=255)                                                   cAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGttt                   >  2:403212/1‑81 (MQ=255)                                                   cAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGtt                    <  2:403161/80‑1 (MQ=255)                                                    aGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGtttt                  >  2:403209/1‑81 (MQ=255)                                                    aGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGttt                   <  2:403162/80‑1 (MQ=255)                                                        gTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTttcttc              <  1:403163/81‑1 (MQ=255)                                                           gCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAgc           >  1:403218/1‑81 (MQ=255)                                                            ccGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAgcg          <  1:403164/81‑1 (MQ=255)                                                              gTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAgcgc         >  1:403195/1‑80 (MQ=255)                                                              gTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAgcgc         >  2:403206/1‑80 (MQ=255)                                                                 tGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGa     >  1:403211/1‑81 (MQ=255)                                                                   aGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGAcc   >  1:403197/1‑81 (MQ=255)                                                                   aGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGAc    <  1:403165/80‑1 (MQ=255)                                                                    gCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCg  <  2:403166/81‑1 (MQ=255)                                                                       |                                                                              AACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCG  >  NC_000913/2260709‑2260856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑