Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,451,016	A→G	D20D (GAT→GAC)	yfcQ ←	putative fimbrial protein YfcQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,451,016	0	A	G	100.0%	111.3 / NA	31	D20D (GAT→GAC)	yfcQ	putative fimbrial protein YfcQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/18);  total (13/18)

GACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCA  >  NC_000913/2450941‑2451088                                                                            |                                                                         gACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTcag                                                                      >  2:438689/1‑80 (MQ=255) gACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTcag                                                                      <  2:438663/80‑1 (MQ=255)   cTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTcagcag                                                                   <  2:438664/81‑1 (MQ=255)       aaTGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCAt                                                                >  1:438698/1‑80 (MQ=255)       aaTGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATg                                                               <  2:438666/81‑1 (MQ=255)       aaTGGTTTTACCACCGCTGATGCTACAGGTTAGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATg                                                               <  2:438665/81‑1 (MQ=255)           gTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGc                                                            >  2:438700/1‑80 (MQ=255)              ttACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTAt                                                         <  2:438667/80‑1 (MQ=255)                accaccGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGgc                                                      >  2:438693/1‑81 (MQ=255)                accaccGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGgc                                                      >  1:438694/1‑81 (MQ=255)                   accGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCt                                                   <  2:438668/81‑1 (MQ=255)                        tGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGa                                              >  2:438697/1‑81 (MQ=255)                             cTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGAcaca                                          <  1:438669/80‑1 (MQ=255)                             cTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACaa                                         <  2:438670/81‑1 (MQ=255)                                cAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACaaa                                        <  2:438671/79‑1 (MQ=255)                                    ttGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAggg                                  >  1:438701/1‑81 (MQ=255)                                       gCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTa                               <  1:438672/81‑1 (MQ=255)                                           tGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGaa                           <  1:438673/81‑1 (MQ=255)                                            gACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGa                            >  1:438706/1‑79 (MQ=255)                                               aataatGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTc                       <  1:438674/81‑1 (MQ=255)                                               aataatGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTc                       >  2:438771/1‑81 (MQ=255)                                                ataatGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCt                      <  1:438675/81‑1 (MQ=255)                                                  aatGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCttt                    >  1:438696/1‑81 (MQ=255)                                                   atGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTtc                   <  1:438676/81‑1 (MQ=255)                                                   atGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTtc                   >  2:438705/1‑81 (MQ=255)                                                    tGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTtct                  >  2:438704/1‑81 (MQ=255)                                                      tCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCa                <  2:438677/81‑1 (MQ=255)                                                        ccGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCAtt              >  1:438703/1‑81 (MQ=255)                                                           tGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGAt           <  2:438678/81‑1 (MQ=255)                                                                 gTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTcca     <  1:438679/81‑1 (MQ=255)                                                                    aTGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTccacca  <  2:438680/81‑1 (MQ=255)                                                                            |                                                                         GACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCA  >  NC_000913/2450941‑2451088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑