Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,693,373	G→C	A724G (GCC→GGC)	purL ←	phosphoribosylformylglycinamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,693,373	0	G	C	100.0%	125.6 / NA	35	A724G (GCC→GGC)	purL	phosphoribosylformylglycinamide synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (21/14);  total (21/14)

GGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCT  >  NC_000913/2693305‑2693448                                                                     |                                                                            ggTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGtt                                                                  >  1:483309/1‑80 (MQ=255)    ggCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACg                                                              >  1:483326/1‑81 (MQ=255)        tGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCtt                                                           >  1:483312/1‑80 (MQ=255)         gccgccgccATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCa                                                         >  1:483325/1‑81 (MQ=255)           cgccgccATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCacc                                                       >  2:483330/1‑81 (MQ=255)               gccATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCaccgacc                                                   <  1:483280/81‑1 (MQ=255)               gccATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCaccgacc                                                   <  1:483279/81‑1 (MQ=255)                ccATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCaccgaccg                                                  >  1:483324/1‑81 (MQ=255)                    ccAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGc                                                 >  1:483322/1‑78 (MQ=255)                     cAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGa                                             >  2:483317/1‑81 (MQ=255)                             ggAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCaga                                     <  2:483281/81‑1 (MQ=255)                                aaGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAggc                                  <  2:483282/81‑1 (MQ=255)                                aaGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAggc                                  >  2:483331/1‑81 (MQ=255)                                aaGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAggc                                  >  2:483319/1‑81 (MQ=255)                                 aGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAggcg                                 >  2:483337/1‑81 (MQ=255)                                  gTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAggcgg                                <  1:483283/81‑1 (MQ=255)                                     tGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGgcggcg                              <  1:483285/80‑1 (MQ=255)                                     tGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGgcggcg                              <  2:483284/80‑1 (MQ=255)                                        tGCGTTTGATATTGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGTCGGCGAAAt                          >  2:483315/1‑81 (MQ=255)                                        tGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAAt                          <  1:483286/81‑1 (MQ=255)                                             ttGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCcag                      >  2:483344/1‑80 (MQ=255)                                             ttGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCcag                      >  2:483379/1‑80 (MQ=255)                                               gATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCcagcag                   >  2:483334/1‑81 (MQ=255)                                                atatCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCagcagc                  <  2:483287/81‑1 (MQ=255)                                                  atCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAgcg                 >  2:483314/1‑80 (MQ=255)                                                      ccAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAAcc            <  2:483289/81‑1 (MQ=255)                                                      ccAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAAcc            <  2:483288/81‑1 (MQ=255)                                                       cAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCg           <  2:483290/81‑1 (MQ=255)                                                       cAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCg           >  2:483321/1‑81 (MQ=255)                                                        aaTTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCgg          >  1:483316/1‑81 (MQ=255)                                                             gtgtTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCa      <  2:483291/80‑1 (MQ=255)                                                              tgtTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCAc     <  2:483292/80‑1 (MQ=255)                                                               gtTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGc   >  2:483329/1‑81 (MQ=255)                                                               gtTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGc   >  1:483340/1‑81 (MQ=255)                                                                ttGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCt  >  1:483323/1‑81 (MQ=255)                                                                     |                                                                            GGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCT  >  NC_000913/2693305‑2693448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑