Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,717,817	C→T	A238T (GCG→ACG)	yfiF ←	putative methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,717,817	0	C	T	100.0%	142.1 / NA	37	A238T (GCG→ACG)	yfiF	putative methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/18);  total (19/18)

CATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCAC  >  NC_000913/2717739‑2717894                                                                               |                                                                              catcCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGtcc                                                                             >  2:487819/1‑81 (MQ=255)       gCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACt                                                                       >  2:487815/1‑81 (MQ=255)       gCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACt                                                                       >  1:487798/1‑81 (MQ=255)       gCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACt                                                                       >  1:487804/1‑81 (MQ=255)         aCGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTcc                                                                     <  1:487760/81‑1 (MQ=255)              aaCAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCcagcag                                                                >  1:487813/1‑81 (MQ=255)              aaCAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCcagcag                                                                >  1:487811/1‑81 (MQ=255)               aCAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGt                                                               <  2:487763/81‑1 (MQ=255)               aCAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGt                                                               <  2:487762/81‑1 (MQ=255)               aCAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGt                                                               <  2:487761/81‑1 (MQ=255)                    gttgtCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGc                                                          >  2:487833/1‑81 (MQ=255)                     ttgtCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCa                                                         <  2:487764/81‑1 (MQ=255)                       gtCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATc                                                       >  1:487814/1‑81 (MQ=255)                             aGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGcac                                                 >  1:487824/1‑81 (MQ=255)                                 aTCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGcaccac                                              <  1:487766/80‑1 (MQ=255)                                 aTCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACg                                             <  2:487765/81‑1 (MQ=255)                                  tCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGa                                            >  2:487810/1‑81 (MQ=255)                                   cGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGa                                            >  1:487823/1‑80 (MQ=255)                                        gAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCtt                                      >  1:487807/1‑81 (MQ=255)                                            gTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCAc                                  <  1:487767/81‑1 (MQ=255)                                             tGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACg                                 <  1:487768/81‑1 (MQ=255)                                               ctctGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGcc                               >  1:487818/1‑81 (MQ=255)                                               ctctGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGc                                <  1:487769/80‑1 (MQ=255)                                                 ctGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCg                              >  1:487845/1‑80 (MQ=255)                                                  tGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCg                              <  1:487770/79‑1 (MQ=255)                                                    cgccgcCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGa                             <  1:487772/78‑1 (MQ=255)                                                    cgccgcCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGt                          <  1:487771/81‑1 (MQ=255)                                                     gccgccTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTg                         >  2:487828/1‑81 (MQ=255)                                                        gccTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgc                      <  1:487773/81‑1 (MQ=255)                                                         ccTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgcg                     >  2:487820/1‑81 (MQ=255)                                                          cTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgcg                     >  2:487842/1‑80 (MQ=255)                                                          cTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgcg                     >  1:487821/1‑80 (MQ=255)                                                                cGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCt                <  1:487775/79‑1 (MQ=255)                                                                cGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCt                <  1:487774/79‑1 (MQ=255)                                                                  gTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTgcgc            <  2:487777/81‑1 (MQ=255)                                                                  gTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTgcgc            <  1:487776/81‑1 (MQ=255)                                                                  gTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTgcg             >  1:487822/1‑80 (MQ=255)                                                                          aGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGc     >  1:487834/1‑80 (MQ=255)                                                                           gccgtcCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGcc    <  1:487778/80‑1 (MQ=255)                                                                            ccgtcCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCAc  <  2:487779/81‑1 (MQ=255)                                                                               |                                                                              CATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCAC  >  NC_000913/2717739‑2717894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑