Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	208,192	T→A	S1023T (TCC→ACC)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	208,192	0	T	A	100.0%	111.5 / NA	29	S1023T (TCC→ACC)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/17);  total (12/17)

CGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACC  >  NC_000913/208115‑208261                                                                              |                                                                      cGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCg                                                                    <  1:41731/81‑1 (MQ=255) cGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCg                                                                    >  1:41774/1‑81 (MQ=255)  ggggCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCgg                                                                   >  2:41770/1‑81 (MQ=255)         gttgttGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCt                                                             <  1:41732/80‑1 (MQ=255)         gttgttGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTg                                                            >  2:41772/1‑81 (MQ=255)            gttgCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTgg                                                           >  1:41778/1‑79 (MQ=255)             ttgCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGaa                                                         <  1:41733/80‑1 (MQ=255)              tgCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGt                                                       >  2:41776/1‑81 (MQ=255)                ccGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTg                                                      <  1:41734/80‑1 (MQ=255)                    gcgGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGAtgtt                                                 >  1:41766/1‑81 (MQ=255)                      gggTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGAtgttgt                                               >  2:41758/1‑81 (MQ=255)                              gTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGATCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGa                                        <  1:41736/80‑1 (MQ=255)                              gTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGAc                                       <  1:41735/81‑1 (MQ=255)                                     aGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTgg                                <  2:41737/81‑1 (MQ=255)                                       cgcgGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGAt                              <  2:41738/81‑1 (MQ=255)                                         cgGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATaa                            <  2:41739/81‑1 (MQ=255)                                         cgGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATa                             >  1:41775/1‑80 (MQ=255)                                          ggCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATaaa                           >  1:41781/1‑81 (MQ=255)                                          ggCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATaaa                           <  2:41740/81‑1 (MQ=255)                                          ggCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATaaa                           >  1:41780/1‑81 (MQ=255)                                             aaTCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATAc                        >  1:41771/1‑81 (MQ=255)                                               tCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCa                      <  1:41741/81‑1 (MQ=255)                                                      ggTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAAtt                <  1:41742/80‑1 (MQ=255)                                                        tATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCt             >  1:853525/1‑81 (MQ=255)                                                              cACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTgg           <  1:41744/77‑1 (MQ=255)                                                              cACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGaa         <  1:41743/79‑1 (MQ=255)                                                                cGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAg     <  1:41745/81‑1 (MQ=255)                                                                     gATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGAcc  <  2:41747/79‑1 (MQ=255)                                                                     gATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGAcc  <  1:41746/79‑1 (MQ=255)                                                                              |                                                                      CGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACC  >  NC_000913/208115‑208261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑