Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,190,185	T→C	L102P (CTT→CCT)	yqiH →	putative fimbrial chaperone YqiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,190,185	0	T	C	100.0%	102.1 / NA	28	L102P (CTT→CCT)	yqiH	putative fimbrial chaperone YqiH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/16);  total (12/16)

TTCCTCCAATGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATG  >  NC_000913/3190110‑3190261                                                                            |                                                                             ttCCTCCAATGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAAcctcct                                                                          >  1:578656/1‑80 (MQ=255)   cctccAATGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTaa                                                                        <  1:578615/80‑1 (MQ=255)     tccAATGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGa                                                                     >  2:578648/1‑81 (MQ=255)      ccAATGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGa                                                                     >  2:578650/1‑80 (MQ=255)       cAATGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACa                                                                   <  1:578616/81‑1 (MQ=255)        aaTGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGa                                                                     >  1:578653/1‑78 (MQ=255)        aaTGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACag                                                                  <  1:578617/81‑1 (MQ=255)        aaTGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACag                                                                  <  1:578618/81‑1 (MQ=255)         aTGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACaga                                                                 <  2:578619/81‑1 (MQ=255)               cGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAAc                                                            <  2:578620/80‑1 (MQ=255)                  ttAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGctc                                                        >  2:578649/1‑81 (MQ=255)                     aaTCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCtctt                                                      <  1:578621/80‑1 (MQ=255)                     aaTCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCtctt                                                      <  2:578622/80‑1 (MQ=255)                      aTCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCtcttct                                                    <  2:578623/81‑1 (MQ=255)                      aTCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCtcttc                                                     >  1:578652/1‑80 (MQ=255)                         ccAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTAt                                                  <  1:578624/80‑1 (MQ=255)                          cAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTAttt                                                <  2:578625/81‑1 (MQ=255)                            gTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTAtttt                                               >  2:578661/1‑80 (MQ=255)                                     ttCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCgag                                     <  1:578626/81‑1 (MQ=255)                                         aaTAAGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAAt                                 <  1:578627/81‑1 (MQ=255)                                            aaGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATAcc                              >  1:578657/1‑81 (MQ=255)                                            aaGAATGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATAcc                              >  2:578654/1‑81 (MQ=255)                                                aTGATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGcc                           >  1:578659/1‑80 (MQ=255)                                                  gATAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCa                         >  1:578658/1‑80 (MQ=255)                                                   aTAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCaa                        >  2:578665/1‑80 (MQ=255)                                                    tAACTCAGGAAAAAATTGCTCAACCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCaaaa                      <  1:578628/81‑1 (MQ=255)                                                                         aaCCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGAt   <  1:578629/79‑1 (MQ=255)                                                                         aaCCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATg  <  2:578630/80‑1 (MQ=255)                                                                         aaCCTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATg  >  2:578664/1‑80 (MQ=255)                                                                            |                                                                             TTCCTCCAATGGTGCGTTTAAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATG  >  NC_000913/3190110‑3190261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑