Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,243,047	A→T	Y90N (TAT→AAT)	uxaA ←	D‑altronate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,243,047	0	A	T	100.0%	121.7 / NA	32	Y90N (TAT→AAT)	uxaA	D‑altronate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (18/14);  total (18/14)

GTTAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGCCAATGCA  >  NC_000913/3242970‑3243123                                                                              |                                                                             gTTAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCt                                                                            >  1:589494/1‑80 (MQ=255)  ttAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGa                                                                          <  2:589446/81‑1 (MQ=255)    aGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGAt                                                                         <  1:589447/80‑1 (MQ=255)    aGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATc                                                                        >  1:589486/1‑81 (MQ=255)       gcgACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATc                                                                        >  2:589479/1‑78 (MQ=255)       gcgACGATAGATCTGCACTCCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAg                                                                     <  2:589448/81‑1 (MQ=255)        cgacgaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGa                                                                    <  2:589449/81‑1 (MQ=255)         gacgaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGAt                                                                   <  2:589450/81‑1 (MQ=255)          acgaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATc                                                                  <  1:589452/81‑1 (MQ=255)          acgaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATc                                                                  <  2:589451/81‑1 (MQ=255)           cgaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCg                                                                 >  1:589485/1‑81 (MQ=255)            gaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGc                                                                >  1:589506/1‑81 (MQ=255)                      cACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCg                                                       <  1:589453/80‑1 (MQ=255)                                   gCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTgg                                         >  1:589492/1‑81 (MQ=255)                                   gCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTgg                                         >  2:589489/1‑81 (MQ=255)                                    ccGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATtg                                            <  1:589454/77‑1 (MQ=255)                                          gcgcAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCTCGTATTGTGGGcgtg                                    <  2:589455/79‑1 (MQ=255)                                             caggcagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacgt                               <  2:589456/81‑1 (MQ=255)                                             caggcagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacgt                               <  2:589457/81‑1 (MQ=255)                                              aggcagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacgt                               >  2:589493/1‑80 (MQ=255)                                                gcagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacgtgaa                            >  2:589501/1‑81 (MQ=255)                                                 cagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacgtgaa                            >  1:589495/1‑80 (MQ=255)                                                 cagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacgtgaa                            >  2:589488/1‑80 (MQ=255)                                                  agATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAaca                          >  1:589484/1‑81 (MQ=255)                                                        tGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGcc                    >  2:589503/1‑81 (MQ=255)                                                                 ggTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATcc           >  1:589496/1‑81 (MQ=255)                                                                 ggTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATcc           >  2:589487/1‑81 (MQ=255)                                                                 ggTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATcc           <  1:589458/81‑1 (MQ=255)                                                                 ggTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATcc           >  1:589500/1‑81 (MQ=255)                                                                       tAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGCCAAt     <  2:589459/81‑1 (MQ=255)                                                                        aGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGCCAATg    >  1:589515/1‑81 (MQ=255)                                                                          cGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGCCAATGCa  >  2:589513/1‑81 (MQ=255)                                                                              |                                                                             GTTAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGCCAATGCA  >  NC_000913/3242970‑3243123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑