Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,863,817	A→T	intergenic (‑214/‑82)	glvC ← / → yidP	putative PTS enzyme II component GlvC/putative DNA‑binding transcriptional regulator YidP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,863,817	0	A	T	100.0%	69.9 / NA	19	intergenic (‑214/‑82)	glvC/yidP	putative PTS enzyme II component GlvC/putative DNA‑binding transcriptional regulator YidP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (7/12);  total (7/12)

CGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTAAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATC  >  NC_000913/3863758‑3863893                                                            |                                                                             cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGtttttt                                                             <  2:714541/77‑1 (MQ=255) cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTc                                                            <  2:714542/78‑1 (MQ=255) cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCaa                                                          <  2:714540/80‑1 (MQ=255) cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAAt                                                         <  2:714543/81‑1 (MQ=255)  gTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATc                                                        >  2:714563/1‑81 (MQ=255)   ttttCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCg                                                       <  1:714544/81‑1 (MQ=255)     ttCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGa                                                      <  1:714545/80‑1 (MQ=255)        gcgAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTaa                                                  <  1:714546/81‑1 (MQ=255)                      ctctGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTtgtgtg                                    >  2:714585/1‑81 (MQ=255)                           tgAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCAt                               >  1:714570/1‑81 (MQ=255)                                acaGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTAccc                          <  2:714547/81‑1 (MQ=255)                                       cacaGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTg                   <  2:714548/81‑1 (MQ=255)                                        acaGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGc                  <  1:714549/81‑1 (MQ=255)                                        acaGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGc                  <  2:714550/81‑1 (MQ=255)                                             aTCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGc              >  1:714568/1‑80 (MQ=255)                                                gAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCaaaa          <  1:714551/81‑1 (MQ=255)                                                gAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCaaa           >  2:714581/1‑80 (MQ=255)                                                        cTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATc  >  1:714577/1‑81 (MQ=255)                                                        cTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATc  >  1:714586/1‑81 (MQ=255)                                                            |                                                                             CGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTAAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATC  >  NC_000913/3863758‑3863893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑