Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,007,952	A→G	K66E (AAA→GAA)	rhtC →	L‑threonine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,007,952	0	A	G	100.0%	95.9 / NA	26	K66E (AAA→GAA)	rhtC	L‑threonine exporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/11);  total (15/11)

TGGGCGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTA  >  NC_000913/4007875‑4008026                                                                              |                                                                           tgGGCGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGaagaaa                                                                         >  2:744820/1‑81 (MQ=255)   ggCGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATg                                                                       <  1:744791/81‑1 (MQ=255)      gTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGcc                                                                    >  2:744834/1‑81 (MQ=255)         cTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTgg                                                                 <  1:744792/81‑1 (MQ=255)                    cTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCt                                                      >  1:744823/1‑81 (MQ=255)                        ggcgTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGAt                                                   >  1:744827/1‑80 (MQ=255)                             aaTGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTAtggt                                             >  2:744821/1‑81 (MQ=255)                               tGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTAtggtgg                                           >  1:744844/1‑81 (MQ=255)                                      ggCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTgg                                    <  1:744793/81‑1 (MQ=255)                                           ggATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGt                               <  1:744794/81‑1 (MQ=255)                                              ttGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTAtc                            <  1:744796/81‑1 (MQ=255)                                              ttGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTAtc                            >  2:744825/1‑81 (MQ=255)                                              ttGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTAtc                            <  1:744795/81‑1 (MQ=255)                                                 cactgctTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATctct                         <  2:744797/79‑1 (MQ=255)                                                      ctTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTctgct                      <  2:744798/79‑1 (MQ=255)                                                        tGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGAt                  >  1:744833/1‑81 (MQ=255)                                                        tGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGAt                  >  1:744835/1‑81 (MQ=255)                                                         ggCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATg                 >  1:744838/1‑81 (MQ=255)                                                           ccTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATggg               >  2:744841/1‑81 (MQ=255)                                                               cATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTa            <  1:744800/80‑1 (MQ=255)                                                               cATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTa            <  2:744799/80‑1 (MQ=255)                                                               cATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTAc           >  2:744837/1‑81 (MQ=255)                                                                   tGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTAc           >  1:744853/1‑77 (MQ=255)                                                                      ttATCGAAGCAATGGCCTGGCTGCATACGCTGATTATGGTTTGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGc    >  2:744852/1‑81 (MQ=255)                                                                        aTCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTa  >  2:744846/1‑81 (MQ=255)                                                                        aTCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTa  >  2:744849/1‑81 (MQ=255)                                                                         tCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTa  <  1:744801/80‑1 (MQ=255)                                                                              |                                                                           TGGGCGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTA  >  NC_000913/4007875‑4008026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑