Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,098,551	A→G	G272G (GGA→GGG)	rhaS →	DNA‑binding transcriptional activator RhaS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,098,551	0	A	G	100.0%	65.2 / NA	19	G272G (GGA→GGG)	rhaS	DNA‑binding transcriptional activator RhaS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/13);  total (6/13)

GATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACTT  >  NC_000913/4098475‑4098621                                                                             |                                                                       gATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCgg                                                                     <  1:763575/80‑1 (MQ=255)   ttCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGAt                                                                  <  1:763577/81‑1 (MQ=255)   ttCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGAt                                                                  <  2:763576/81‑1 (MQ=255)   ttCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGAt                                                                  <  1:763578/81‑1 (MQ=255)     cAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATgg                                                                >  2:763607/1‑81 (MQ=255)     cAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATg                                                                 <  1:763579/80‑1 (MQ=255)     cAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATg                                                                 <  1:763580/80‑1 (MQ=255)      aGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGc                                                               >  2:763612/1‑81 (MQ=255)                       ttCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAAcgc                                                <  2:763581/79‑1 (MQ=255)                        tCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGa                                              <  2:763582/80‑1 (MQ=255)                          gACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAAtc                                           >  1:763605/1‑81 (MQ=255)                                   tCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAAc                                   <  1:763583/80‑1 (MQ=255)                                                 aCTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCa                     <  2:763584/80‑1 (MQ=255)                                                       cACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGc               >  2:763610/1‑80 (MQ=255)                                                        aCCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGa             <  2:763585/81‑1 (MQ=255)                                                         ccGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGaa            <  1:763587/81‑1 (MQ=255)                                                         ccGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGaa            <  2:763586/81‑1 (MQ=255)                                                           gcgTGATATTCGCCAGGGGCGGGTTGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGaat           >  2:763617/1‑80 (MQ=255)                                                                   ttCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACtt  >  1:763615/1‑81 (MQ=255)                                                                             |                                                                       GATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACTT  >  NC_000913/4098475‑4098621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑