Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,371,109	G→A	V29I (GTT→ATT)	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,371,109	0	G	A	96.3%	99.2 / NA	27	V29I (GTT→ATT)	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (18/8);  minor base T (1/0);  total (19/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAGTTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGGTGTT  >  NC_000913/4371035‑4371186                                                                           |                                                                              aaGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTAc                                                                           <  1:820190/79‑1 (MQ=255)   gACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTc                                                                       >  1:820222/1‑81 (MQ=255)        aaaaTTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTc                                                                   >  1:820214/1‑80 (MQ=255)            ttCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCaaaa                                                              <  2:820191/81‑1 (MQ=255)               ggTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGTAGTGAAAATTACCCTCGGTCCAAAAGGc                                                           >  1:820227/1‑81 (MQ=255)                   acgacgCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGt                                                        >  1:820217/1‑80 (MQ=255)                   acgacgCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGt                                                        >  1:820225/1‑80 (MQ=255)                     gacgCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAAc                                                     >  1:820226/1‑81 (MQ=255)                        gCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACg                                                    >  2:820223/1‑79 (MQ=255)                          tCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGACATTTACCCTCGGTCCAAAAGGCCGTAACGTAGt                                                >  1:820229/1‑81 (MQ=255)                            gtgtGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTc                                              >  2:820221/1‑81 (MQ=255)                             tgtgAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCt                                             >  2:820236/1‑81 (MQ=255)                                   aaTGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATaa                                       <  1:820192/81‑1 (MQ=255)                                       ctgcGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCt                                   <  1:820193/81‑1 (MQ=255)                                          cgcgGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCtt                                  <  1:820194/79‑1 (MQ=255)                                             ggcgTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGt                             >  2:820230/1‑81 (MQ=255)                                              gcgTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTg                            >  2:820232/1‑81 (MQ=255)                                                gTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCa                          >  1:820228/1‑81 (MQ=255)                                                      gTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGAc                     >  1:820250/1‑80 (MQ=255)                                                      gTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGAc                     >  2:820219/1‑80 (MQ=255)                                                      gTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGAc                     >  2:820237/1‑80 (MQ=255)                                                         cTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCAt                  >  1:820235/1‑80 (MQ=255)                                                              aGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCaa            <  1:820195/81‑1 (MQ=255)                                                                   cAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAATGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGAt        <  1:820196/80‑1 (MQ=255)                                                                     gTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGgt     <  2:820197/81‑1 (MQ=255)                                                                       gAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGgtgt   >  2:820242/1‑81 (MQ=255)                                                                       gAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGgtgt   >  2:820244/1‑81 (MQ=255)                                                                        aaaaTTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGgtgt   <  2:820198/80‑1 (MQ=255)                                                                        aaaaTTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGGTGtt  <  1:820199/81‑1 (MQ=255)                                                                           |                                                                              AAGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAGTTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGGTGTT  >  NC_000913/4371035‑4371186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑