Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	721,886	(C)5→4	coding (2529/2685 nt)	kdpD ←	sensory histidine kinase KdpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	721,882	0	C	.	100.0%	46.0 / NA	12	coding (2533/2685 nt)	kdpD	sensory histidine kinase KdpD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (8/4);  total (8/4)

CCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCCCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTACCCCCGGTACTGCCGACTCTTTATTCCCGCGAGCAAACTTATCAAATATCGTCT  >  NC_000913/721803‑721935                                                                                |                                                      ccTTCCGGTCGGTTGAACGCGGTAATAGTGCCCCCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTAcc                                                      <  1:139781/81‑1 (MQ=255)      cggtcggtTGAACGCGGTAATAGTGCCCCCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTAccccgg                                                  <  2:139782/80‑3 (MQ=255)        gtcggtTGAACGCGGTAATAGTGCCCCCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTA‑CCCCGGTAc                                              <  2:139783/81‑1 (MQ=255)          cggtTGAACGCGGTAATAGTGCCCCCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTA‑CCCCGGTACTg                                            >  1:139811/1‑81 (MQ=255)             ttGAACGCGGTAATAGTGCCCCCGTGTACATCCACTATCGTCCGACAAATTGCCAGTCCAAGCCCTA‑CCCCGGTACTGcc                                          >  2:139806/1‑80 (MQ=255)             ttGAACGCGGTAATAGTGCCCCCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTA‑CCCCGGTACTGcc                                          >  1:139823/1‑80 (MQ=255)              tGAACGCGGTAATAGTGCCCCCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTA‑CCCCGGTACTGCCGa                                        >  2:139796/1‑81 (MQ=255)               gAACGCGGTAATAGTGCCCCCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTA‑CCCCGGTACTGCCGa                                        <  2:139784/80‑1 (MQ=255)               gAACGCGGTAATAGTGCCCCCGTGTACATCCACTACCGCCCGACAAATTGCCAGTCCAAGCCCTA‑CCCCGGTACTGCCTAc                                       >  2:139812/1‑81 (MQ=255)                aaCGCGGTAATAGTGCCCCCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTA‑CCCCGGTACTGCCGAct                                      >  1:139805/1‑81 (MQ=255)                         aTAGTGCCCCCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTA‑CCCCGGTACTGCCGACTCTTTATTc                              <  1:139785/80‑1 (MQ=255)                                       tACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTA‑CCCCGGTACTGCCGACTCTTTATTCCCGCGAGCAAACtt                <  2:139786/80‑1 (MQ=255)                                            ccACTATCGCCCGACAAATTGCCAGTCCAAGCCCTA‑CCCCGGTACTGCCGACTCTTTATTCCCGCGAGCAAACTTATCaaa          >  1:139815/1‑81 (MQ=255)                                                    gCCCGACAAATTGCCAGTCCAAGCCCTA‑CCCCGGTACTGCCGACTCTTTATTCCCGCGAGCAAACTTATCAAATATCGTCt  >  2:139807/1‑81 (MQ=255)                                                                                |                                                      CCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCCCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTACCCCCGGTACTGCCGACTCTTTATTCCCGCGAGCAAACTTATCAAATATCGTCT  >  NC_000913/721803‑721935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑