Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	723,868	(G)5→4	coding (547/2685 nt)	kdpD ←	sensory histidine kinase KdpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	723,864	0	G	.	100.0%	86.7 / NA	21	coding (551/2685 nt)	kdpD	sensory histidine kinase KdpD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (7/14);  total (7/14)

AAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCCGGGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAAT  >  NC_000913/723786‑723937                                                                               |                                                                          aaaaaaTGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCCggg                                                                         >  2:140162/1‑81 (MQ=255)    aaaTGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCCggggc                                                                       >  1:140171/1‑79 (MQ=255)       tGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGt                                                                  <  2:140142/81‑1 (MQ=255)        gTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTc                                                                 >  1:140164/1‑81 (MQ=255)           cAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTccac                                                              >  1:140178/1‑81 (MQ=255)                         gCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCAcgtcgt                                                >  1:140170/1‑81 (MQ=255)                          ccTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACgtcgtc                                               <  1:140143/81‑1 (MQ=255)                          ccTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACgtcgtc                                               <  2:613992/81‑1 (MQ=255)                           cTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGtcgtcg                                              <  2:140144/81‑1 (MQ=255)                           cTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGtcgtcg                                              <  1:584432/81‑1 (MQ=255)                              cccGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GCGGCAAGTCCACCAGCACCACGTCGTcggc                                            <  2:140145/80‑1 (MQ=255)                                cGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCggcggc                                         <  1:140146/81‑1 (MQ=255)                                  gCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCAt                                       <  2:140147/81‑1 (MQ=255)                                   cAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATc                                      >  2:140169/1‑81 (MQ=255)                                      tataGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGaa                                   <  1:140148/81‑1 (MQ=255)                                        taGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGaaaa                                 <  1:140149/81‑1 (MQ=255)                                                  cttcttTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCggg                       >  1:140182/1‑81 (MQ=255)                                                         cAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGt                 <  2:140150/80‑1 (MQ=255)                                                          aGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGttt               >  1:140175/1‑81 (MQ=255)                                                             cGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTccc            >  2:140179/1‑81 (MQ=255)                                                                  gcgcAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTAcc       <  2:140151/81‑1 (MQ=255)                                                                   cgcAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCt      <  1:140152/81‑1 (MQ=255)                                                                       gATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAAt  <  1:140153/81‑1 (MQ=255)                                                                               |                                                                          AAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCCGGGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAAT  >  NC_000913/723786‑723937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑