Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	742,314	(C)5→4	coding (243/1482 nt)	dtpD ←	dipeptide:H(+) symporter DtpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	742,310	0	C	.	100.0%	94.2 / NA	23	coding (247/1482 nt)	dtpD	dipeptide:H(+) symporter DtpD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (7/16);  total (7/16)

AATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATA  >  NC_000913/742230‑742382                                                                                 |                                                                         aaTCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGc                                                                          >  1:143245/1‑81 (MQ=255)  aTCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGc                                                                          <  1:143190/80‑1 (MQ=255)   tCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGccc                                                                        <  2:143191/81‑1 (MQ=255)     gCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGccc                                                                        >  2:143240/1‑79 (MQ=255)       cAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGccccag                                                                     >  2:143233/1‑78 (MQ=255)         gggACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCAt                                                                 <  1:143192/81‑1 (MQ=255)          ggACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATc                                                                <  1:143193/81‑1 (MQ=255)           gACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCa                                                               >  1:143235/1‑81 (MQ=255)               gATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGc                                                           <  1:143194/81‑1 (MQ=255)                   gagGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCAt                                                        <  1:143195/80‑1 (MQ=255)                    agGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGc                                                      <  2:143196/81‑1 (MQ=255)                      gAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGa                                                    >  2:143237/1‑81 (MQ=255)                           aCGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGcc                                               <  1:143197/81‑1 (MQ=255)                           aCGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGc                                                <  2:143198/80‑1 (MQ=255)                              gatggatCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCgag                                            <  1:143199/81‑1 (MQ=255)                               atggatCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCgaga                                           <  2:143200/81‑1 (MQ=255)                                     ctcACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTa                                     >  2:143232/1‑81 (MQ=255)                                        aCTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACttt                                      <  2:143202/77‑1 (MQ=255)                                        aCTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATcc                                  <  2:143201/81‑1 (MQ=255)                                            gCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGc                                <  2:143203/79‑1 (MQ=255)                                                   gCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGcc                       <  2:143204/81‑1 (MQ=255)                                                          aTGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCTACAAAAAGCCACCGAg                 >  1:143248/1‑80 (MQ=255)                                                          aTGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAg                 >  1:143241/1‑80 (MQ=255)                                                                gATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGc          >  1:143238/1‑81 (MQ=255)                                                                       aTCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACa    >  1:143244/1‑80 (MQ=255)                                                                       aTCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACAt   <  2:143205/81‑1 (MQ=255)                                                                       aTCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACAt   <  2:143206/81‑1 (MQ=255)                                                                        tCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATa  <  1:143207/81‑1 (MQ=255)                                                                                 |                                                                         AATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATA  >  NC_000913/742230‑742382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑