Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	868,422	(C)8→7	coding (903/1872 nt)	gsiA →	glutathione ABC transporter ATP binding subunit GsiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	868,415	0	C	.	100.0%	121.7 / NA	29	coding (896/1872 nt)	gsiA	glutathione ABC transporter ATP binding subunit GsiA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (16/13);  total (16/13)

GCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCC  >  NC_000913/868338‑868483                                                                              |                                                                     gCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGcccc                                                                   <  1:164844/81‑1 (MQ=255)         gTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGcccccccatc                                                             >  2:164888/1‑76 (MQ=255)          tGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAg                                                         <  1:164845/81‑1 (MQ=255)           gCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAg                                                         >  1:164895/1‑80 (MQ=255)             gATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAg                                                         >  2:164876/1‑78 (MQ=255)                  aaGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAAc                                                 >  1:164900/1‑81 (MQ=255)                  aaGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAAc                                                 >  1:164879/1‑81 (MQ=255)                     ggTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACggt                                              <  2:164846/81‑1 (MQ=255)                          gATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGtggtgg                                         <  1:164847/81‑1 (MQ=255)                            ttATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGAt                                       <  1:164848/81‑1 (MQ=255)                               tCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGc                                    <  1:164849/81‑1 (MQ=255)                                ccacGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGGAACAGG‑CCCCCCCATCGAGCAGAAAGCGGTGGTGGATGGc                                    <  2:164850/77‑1 (MQ=255)                                 cccGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGa                                  <  2:164851/81‑1 (MQ=255)                                    gACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                               >  1:164890/1‑81 (MQ=255)                                       gTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGt                            >  1:164896/1‑81 (MQ=255)                                        tttCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGtt                           >  1:164886/1‑81 (MQ=255)                                          tCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGttt                          >  2:164887/1‑80 (MQ=255)                                          tCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGttt                          >  1:164904/1‑80 (MQ=255)                                            ccGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTAc                       <  2:164852/81‑1 (MQ=255)                                                 gATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGt                   <  1:164853/80‑1 (MQ=255)                                                  atatCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGc                 >  2:164884/1‑81 (MQ=255)                                                     tCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGt               <  1:164855/80‑1 (MQ=255)                                                     tCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGt               <  1:164854/80‑1 (MQ=255)                                                       gCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAAt            >  1:164889/1‑81 (MQ=255)                                                          tGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCtt         >  2:164905/1‑81 (MQ=255)                                                          tGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCtt         >  2:164885/1‑81 (MQ=255)                                                            aaCATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGt       >  2:164901/1‑81 (MQ=255)                                                             aCATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGt       >  2:164906/1‑80 (MQ=255)                                                             aCATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGt       >  2:164891/1‑80 (MQ=255)                                                             aCATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTc      <  2:164856/81‑1 (MQ=255)                                                                  cAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCAccc  <  1:164857/80‑1 (MQ=255)                                                                              |                                                                     GCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCC  >  NC_000913/868338‑868483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑