Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	911,171	Δ1 bp	intergenic (‑122/+11)	poxB ← / ← hcr	pyruvate oxidase/NADH oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	911,169	0	G	.	100.0%	147.3 / NA	35	intergenic (‑120/+13)	poxB/hcr	pyruvate oxidase/NADH oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (20/15);  total (20/15)

AGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATT  >  NC_000913/911089‑911245                                                                                 |                                                                             aGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCg                                                                              >  1:171691/1‑81 (MQ=255)        cTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGACCGATGAAAGGAATATCATC‑GGCATAAg                                                                      >  2:171700/1‑81 (MQ=255)             ggAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGAt                                                                 <  1:171665/81‑1 (MQ=255)               aGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTa                                                               <  1:171666/81‑1 (MQ=255)                gggggAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTAt                                                              <  2:171667/81‑1 (MQ=255)                   ggAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGc                                                            >  1:171717/1‑80 (MQ=255)                   ggAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGc                                                            >  2:171703/1‑80 (MQ=255)                   ggAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGc                                                            >  1:171690/1‑80 (MQ=255)                       gggATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGaa                                                       >  2:171699/1‑81 (MQ=255)                          aTGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCGCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAAcc                                                     >  2:171692/1‑80 (MQ=255)                          aTGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAAcc                                                     >  2:171698/1‑80 (MQ=255)                              gagagaAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATc                                                >  2:171704/1‑81 (MQ=255)                                   aaaGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCt                                           >  1:171719/1‑81 (MQ=255)                                    aaGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTg                                          >  1:171708/1‑81 (MQ=255)                                    aaGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTg                                          <  2:171668/81‑1 (MQ=255)                                     aggaggCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGc                                         <  2:171669/81‑1 (MQ=255)                                     aggaggCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGc                                         <  1:171670/81‑1 (MQ=255)                                       gaggCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCgg                                       <  2:171671/81‑1 (MQ=255)                                            aCTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATgg                                   >  1:171709/1‑80 (MQ=255)                                                aCGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAg                                >  1:171718/1‑79 (MQ=255)                                                aCGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAg                                >  1:171706/1‑79 (MQ=255)                                                  ggTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGc                            <  2:171672/81‑1 (MQ=255)                                                       aaTAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGcc                       <  2:171673/81‑1 (MQ=255)                                                        aTAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGc                        >  1:171701/1‑79 (MQ=255)                                                         tAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAg                     <  1:171674/81‑1 (MQ=255)                                                          aGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAg                     >  2:171734/1‑80 (MQ=255)                                                           gCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTa                   >  1:171702/1‑81 (MQ=255)                                                            cccGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTAc                  <  1:171675/81‑1 (MQ=255)                                                                aTGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTaa              >  1:171711/1‑81 (MQ=255)                                                                  gAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAAcc            >  1:171716/1‑81 (MQ=255)                                                                     aGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTc         <  1:171676/81‑1 (MQ=255)                                                                      ggAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCtt          >  2:171707/1‑79 (MQ=255)                                                                      ggAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCa        <  2:171677/81‑1 (MQ=255)                                                                      ggAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCa        <  1:171678/81‑1 (MQ=255)                                                                        aaTATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGc      >  2:171720/1‑81 (MQ=255)                                                                            tcatc‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGAtt  <  1:171679/81‑1 (MQ=255)                                                                                 |                                                                             AGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATT  >  NC_000913/911089‑911245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑