Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,135,698	Δ1 bp	coding (135/699 nt)	flgH →	flagellar L‑ring protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,135,696	0	C	.	100.0%	116.1 / NA	28	coding (133/699 nt)	flgH	flagellar L‑ring protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (10/18);  total (10/18)

TAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGTTTGAAGATCGTCG  >  NC_000913/1135619‑1135772                                                                              |                                                                             tAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGccgt                                                                           >  1:206169/1‑79 (MQ=255)     cGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGcc                                                                      <  2:206130/81‑1 (MQ=255)      ggCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCa                                                                     <  2:206131/81‑1 (MQ=255)           cgcCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACgg                                                                 <  1:206132/80‑1 (MQ=255)                 gATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTa                                                            <  2:206133/79‑1 (MQ=255)                 gATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTAtt                                                          <  1:206134/81‑1 (MQ=255)                 gATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTAtt                                                          >  2:206176/1‑81 (MQ=255)                          cACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGt                                                   <  2:206135/79‑1 (MQ=255)                            cgccgcTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGc                                               <  2:206136/81‑1 (MQ=255)                              ccgcTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCt                                              >  1:206182/1‑80 (MQ=255)                               cgcTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCt                                              >  2:206173/1‑79 (MQ=255)                                gcTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAg                                           <  1:206137/81‑1 (MQ=255)                                 cTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGc                                          <  2:206138/81‑1 (MQ=255)                                    gTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGa                                       <  2:206139/81‑1 (MQ=255)                                     tGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGAt                                      <  1:206141/81‑1 (MQ=255)                                     tGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGAt                                      <  1:206140/81‑1 (MQ=255)                                       cAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTa                                    <  2:206142/81‑1 (MQ=255)                                         gggggCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAAc                                  >  2:206174/1‑81 (MQ=255)                                          ggggCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACt                                 <  2:206143/81‑1 (MQ=255)                                           gggCGACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTa                                <  2:206144/81‑1 (MQ=255)                                            ggCGACCAGTGCACAACCGGATCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTAt                               <  1:206145/81‑1 (MQ=255)                                               gACCAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGc                            >  1:206181/1‑81 (MQ=255)                                                 ccAGTGCACAACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTa                          >  1:206185/1‑81 (MQ=255)                                                       cacaACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAAcc                    >  1:206192/1‑81 (MQ=255)                                                        acaACCGGTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCg                   <  2:206146/81‑1 (MQ=255)                                                              ggTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGttt             >  2:206178/1‑81 (MQ=255)                                                              ggTTCCCGGTCCGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGttt             >  2:206179/1‑81 (MQ=255)                                                                        ccGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGTTTGAAGAtcgtc   >  1:206183/1‑81 (MQ=255)                                                                         cGACG‑CCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGTTTGAAGAtcgtcg  <  1:206147/81‑1 (MQ=255)                                                                              |                                                                             TAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGTTTGAAGATCGTCG  >  NC_000913/1135619‑1135772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑