Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,146,603	(T)7→6	noncoding (15/169 nt)	sraB →	small RNA SraB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,146,597	0	T	.	100.0%	66.9 / NA	17	noncoding (9/169 nt)	sraB	small RNA SraB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (7/10);  total (7/10)

AAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGT  >  NC_000913/1146524‑1146672                                                                          |                                                                            aaaaGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATAttttttc                                                                       >  1:207908/1‑79 (MQ=255)               gTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCg                                                        >  2:207907/1‑80 (MQ=255)                           gagCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGc                                           >  2:207905/1‑81 (MQ=255)                            agCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGcc                                          <  2:207880/81‑1 (MQ=255)                             gcgcGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCa                                         <  1:207881/81‑1 (MQ=255)                              cgcgACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCAt                                        <  2:207882/81‑1 (MQ=255)                              cgcgACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCAt                                        >  2:504153/1‑81 (MQ=255)                               gcgACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCAtt                                       >  1:207910/1‑81 (MQ=255)                                 gACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCAtttt                                     <  1:207883/81‑1 (MQ=255)                                          gCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATgc                            <  1:207884/81‑1 (MQ=255)                                           cGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATgcg                           >  2:207909/1‑81 (MQ=255)                                                tGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCg                      <  1:207886/81‑1 (MQ=255)                                                tGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCg                      <  1:207885/81‑1 (MQ=255)                                                  gAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGtt                    >  2:207903/1‑81 (MQ=255)                                                           aTAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGtt           <  2:207887/81‑1 (MQ=255)                                                             aaGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGa         >  2:207906/1‑81 (MQ=255)                                                                   aGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGAtgttt    <  1:207888/80‑1 (MQ=255)                                                                    ggCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGAtgtttgt  <  2:207889/81‑1 (MQ=255)                                                                          |                                                                            AAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGT  >  NC_000913/1146524‑1146672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑