Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,185,245	(T)6→5	coding (350/1137 nt)	potA ←	spermidine preferential ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,185,240	0	T	.	100.0%	81.4 / NA	20	coding (355/1137 nt)	potA	spermidine preferential ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (8/12);  total (8/12)

CGGTTTGCGTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTG  >  NC_000913/1185160‑1185309                                                                                 |                                                                      cGGTTTGCGTTGAGCGAAGGTTTCCAACTGCACCATCCGTAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGt                                                                       >  2:213613/1‑81 (MQ=255)  ggTTTGCGTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGtt                                                                      <  1:213582/81‑1 (MQ=255)    tttGCGTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGtttt                                                                    <  2:213583/81‑1 (MQ=255)       gCGTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGtttttg                                                                  >  1:213624/1‑79 (MQ=255)          ttGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATg                                                             <  1:213584/81‑1 (MQ=255)            gAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATgc                                                            <  1:213585/80‑1 (MQ=255)            gAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATgc                                                            <  1:213586/80‑1 (MQ=255)              gCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATgcgc                                                          >  2:213616/1‑80 (MQ=255)               cGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCaa                                                        <  1:213587/81‑1 (MQ=255)                    gTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCaa                                                   <  2:213588/81‑1 (MQ=255)                         cAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGc                                               <  2:213589/80‑1 (MQ=255)                                aCCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACAtttt                                       >  1:213615/1‑81 (MQ=255)                                      cGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAaca                                 <  1:213590/81‑1 (MQ=255)                                        cAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACg                               <  1:213591/81‑1 (MQ=255)                                         aGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATCCGCAACCCAAAGGCCACATTTTCGAACACgg                              >  2:213626/1‑81 (MQ=255)                                                    cACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTgggg                    >  2:213625/1‑80 (MQ=255)                                                      cgcgGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGa                  >  2:213619/1‑80 (MQ=255)                                                        cgGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGaa                 >  1:213620/1‑79 (MQ=255)                                                              gTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCg          >  1:213622/1‑80 (MQ=255)                                                              gTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGt         <  2:213592/81‑1 (MQ=255)                                                              gTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGt         <  1:213593/81‑1 (MQ=255)                                                               tAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCGACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTa        <  2:213594/81‑1 (MQ=255)                                                                 aTTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGc      <  1:213595/81‑1 (MQ=255)                                                                      agcagcGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTg  >  2:213630/1‑80 (MQ=255)                                                                                 |                                                                      CGGTTTGCGTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTG  >  NC_000913/1185160‑1185309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑