Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,197,711	Δ1 bp	coding (527/705 nt)	ymfE ←	e14 prophage; uncharacterized protein YmfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,197,709	0	T	.	100.0%	19.0 / NA	6	coding (529/705 nt)	ymfE	e14 prophage; uncharacterized protein YmfE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (2/4);  total (2/4)

TACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAG  >  NC_000913/1197634‑1197782                                                                            |                                                                          taCCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCAttgttt                                                                      <  2:215328/81‑5 (MQ=255)              gaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCA‑TTGTTTGCAAAAAAAGat                                                         >  1:215336/1‑80 (MQ=255)                                aaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCA‑TTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAAt                                      >  2:215337/1‑81 (MQ=255)                                          gACAGCAAAGAACCTTCAACATGAAAAATATCCA‑TTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCg                            <  2:215329/81‑1 (MQ=255)                                            cAGCAAAGAACCTTCAACATGAAAAATATCCA‑TTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGaa                          <  2:215330/81‑1 (MQ=255)                                                                 aaaaaTATCCA‑TTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa      <  2:215331/80‑1 (MQ=255)                                                                    aaTATCCA‑TTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAg  <  2:215332/81‑1 (MQ=255)                                                                            |                                                                          TACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAG  >  NC_000913/1197634‑1197782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑