Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,255	(G)5→4	noncoding (74/85 nt)	tyrV ←	tRNA‑Tyr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,251	0	G	.	100.0%	68.0 / NA	17	noncoding (78/85 nt)	tyrV	tRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (9/8);  total (9/8)

ATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGA  >  NC_000913/1287175‑1287320                                                                             |                                                                      aTCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTgggg                                                                    <  2:227208/80‑1 (MQ=255)  tCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggaa                                                                  <  2:227210/81‑3 (MQ=255)  tCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggaa                                                                  <  2:227209/81‑3 (MQ=255)    cTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggaag                                                                 >  1:227235/1‑77 (MQ=255)             aTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGT‑GGGGAAGGATTCGAAcc                                                      >  2:227231/1‑81 (MQ=255)             aTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGT‑GGGGAAGGATTCGAAcc                                                      >  1:227236/1‑81 (MQ=255)                  cAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGT‑GGGGAAGGATTCGAACCTTCGa                                                 <  1:227211/81‑1 (MQ=255)                       tatTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGT‑GGGGAAGGATTCGAACCTTCGAAGTCg                                            <  2:227212/81‑1 (MQ=255)                             aTTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGT‑GGGGAAGGATTCGAACCTTCGAAGTCGATGAc                                       <  2:227213/80‑1 (MQ=255)                                gCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGT‑GGGGAAGGATTCGAACCTTCGAAGTCGATGACgg                                     >  2:227246/1‑79 (MQ=255)                                gCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGT‑GGGGAAGGATTCGAACCTTCGAAGTCGATGACGGc                                    <  2:227214/80‑1 (MQ=255)                                 cGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGT‑GGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa                                   <  1:227215/80‑1 (MQ=255)                                   gATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGT‑GGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAt                                <  1:227216/81‑1 (MQ=255)                                    aTTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGT‑GGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAtt                               >  2:227237/1‑81 (MQ=255)                                       cGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGT‑GGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTa                             >  1:227239/1‑80 (MQ=255)                                           gggAAGTTCAGGGACTTTTGAAAGTGATGGTGGT‑GGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc                        >  2:227238/1‑81 (MQ=255)                                             gAAGTTCAGGGACTTTTGAAAGTGATGGTGGT‑GGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg                      <  2:227217/81‑1 (MQ=255)                                                               aaaGTGATGGTGGT‑GGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCgg    >  1:227240/1‑81 (MQ=255)                                                                aaGTGATGGTGGT‑GGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCgg    >  1:227244/1‑80 (MQ=255)                                                                aaGTGATGGTGGT‑GGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCgg    >  1:227241/1‑80 (MQ=255)                                                                 aGTGATGGTGGT‑GGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGa  <  1:227218/81‑1 (MQ=255)                                                                             |                                                                      ATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGA  >  NC_000913/1287175‑1287320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑