| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,314,871 | (T)6→5 | coding (354/507 nt) | yciE ← | DUF892 domain‑containing protein YciE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,314,866 | 0 | T | . | 91.7% | 35.7 / ‑0.9 | 12 | coding (359/507 nt) | yciE | DUF892 domain‑containing protein YciE |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (5/6); total (6/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAA > NC_000913/1314790‑1314932 | aaTCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCAttt < 1:230788/79‑1 (MQ=255) cAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCAtttttgct > 2:230810/1‑78 (MQ=255) cAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCAtttttgct > 2:230816/1‑78 (MQ=255) cTTTTCCTCATTTAAAATCGCTTCGCTGGTTGGAATTGAAGCTGTATCACCGGTATTTTTT‑CTGCTGCTAATAGTGAGgtg > 2:230818/1‑81 (MQ=255) aTTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGc > 2:230807/1‑80 (MQ=255) aaaaTCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGAtt < 2:230789/80‑1 (MQ=255) aaaTCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGAttt < 2:230790/80‑1 (MQ=255) aTCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCaa < 2:230791/81‑1 (MQ=255) tCGATGGTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCt > 2:230812/1‑81 (MQ=255) cGATGGTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCt < 2:230792/80‑1 (MQ=255) cGATGGTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTc > 1:230813/1‑81 (MQ=255) gATGGTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCg < 1:230793/81‑1 (MQ=255) tGGTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGaa < 2:230794/81‑1 (MQ=255) cTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTaa > 1:230815/1‑80 (MQ=255) gTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTaa > 1:230819/1‑78 (MQ=255) | AATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCTAA > NC_000913/1314790‑1314932 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |