| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,346,840 | (T)8→7 | coding (45/105 nt) | ymiB → | putative protein YmiB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,346,833 | 0 | T | . | 93.3% | 49.6 / ‑1.7 | 15 | coding (38/105 nt) | ymiB | putative protein YmiB |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (10/4); total (11/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
CTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATC > NC_000913/1346753‑1346906 | ctccGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGt < 2:234999/81‑1 (MQ=255) ggTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGttttt < 1:235000/81‑1 (MQ=255) aCAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATTACTTACATGAAATTAACGGCGGCTAAACGCATTGtttttttata < 1:235001/81‑4 (MQ=255) ggTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAAt > 1:235015/1‑81 (MQ=255) ggTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAAt < 1:235002/81‑1 (MQ=255) aataaaAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGt > 1:235021/1‑81 (MQ=255) ggAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTCTTATATATTTATTTGTAATCCAGTTTTGGa > 2:235027/1‑80 (MQ=255) gAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGaa > 2:235018/1‑80 (MQ=255) acaTGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGc < 1:235003/81‑1 (MQ=255) aTGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATACTTATTTGTAATCCAGTTTTGGAAAAACGc > 2:235014/1‑79 (MQ=255) tACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGtttt > 1:235022/1‑80 (MQ=255) aaaTTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGa < 2:235004/81‑1 (MQ=255) aTTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGaaa < 1:235005/81‑1 (MQ=255) cggcTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGtt > 1:235020/1‑80 (MQ=255) ggcTAAACGCATTGTTTTTTTT‑TATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAGGTCAGTTa > 2:235026/1‑80 (MQ=255) ggcTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTa > 2:235019/1‑80 (MQ=255) aaaCGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTaaaaaa > 2:235023/1‑81 (MQ=255) aCGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATc > 2:235017/1‑81 (MQ=255) | CTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATC > NC_000913/1346753‑1346906 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |