Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,357,678	Δ1 bp	intergenic (‑10/+124)	ymjA ← / ← puuP	DUF2543 domain‑containing protein YmjA/putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,357,675	0	C	.	100.0%	53.6 / NA	14	intergenic (‑7/+127)	ymjA/puuP	DUF2543 domain‑containing protein YmjA/putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (11/3);  total (11/3)

CGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCGTGATCCGGGTCATA  >  NC_000913/1357596‑1357734                                                                                |                                                            cGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTGc                                                             <  2:236488/80‑1 (MQ=255)   aCAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTGccct                                                          <  1:236489/81‑2 (MQ=255)      ggCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTGccctggg                                                       >  1:236507/1‑77 (MQ=255)      ggCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTGccctggg                                                       >  1:236514/1‑77 (MQ=255)          ctGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTG‑CCCTGGGGtt                                                   >  1:236510/1‑80 (MQ=255)                        gcgTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTG‑CCCTGGGGTTAATGCGCTAAGCAt                                     >  1:236506/1‑80 (MQ=255)                          gTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTG‑CCCTGGGGTTAATGCGCTAAGCATAAg                                  <  1:236490/81‑1 (MQ=255)                            aCTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTG‑CCCTGGGGTTAATGCGCTAAGCATAAggg                                >  1:236517/1‑81 (MQ=255)                               cATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTG‑CCCTGGGGTTAATGCGCTAAGCATAAGGGCg                              >  2:236519/1‑80 (MQ=255)                                    gCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTG‑CCCTGGGGTTAATGCGCTAAGCATAAGGGCGgatga                         >  2:236516/1‑80 (MQ=255)                                         atCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTG‑CCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCAc                   >  2:236513/1‑81 (MQ=255)                                           cAAAATATTTTAGCGGGATATCGTGGTTCATTATTTG‑CCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTc                 >  2:236524/1‑81 (MQ=255)                                              aaTATTTTAGCGGGATATCGTGGTTCATTATTTG‑CCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCGTg              <  2:236491/81‑1 (MQ=255)                                               atatTTTAGCGGGATATCGTGGTTCATTATTTG‑CCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCGTGa             >  1:236528/1‑81 (MQ=255)                                                tatTTTAGCGGGATATCGTGGTTCATTATTTG‑CCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCGTGAt            <  2:236492/81‑1 (MQ=255)                                                   tttAGCGGGATATCGTGGTTCATTATTTG‑CCCTGGGGTTAATTCGCTAAGCATAAGGGCGGATGATAGCACTCGTGATCCg         >  2:236521/1‑81 (MQ=255)                                                        cGGGATATCGTGGTTCATTATTTG‑CCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCGTGATCCGGGTCa    >  1:236530/1‑81 (MQ=255)                                                           gttatCGTGGTTCATTATTCG‑TCCTGGGGTTAATGCGCTAAGCATAAGGCCGGATGATAGCACTCGTGGTCCGGGTCATa  >  2:236536/3‑80 (MQ=255)                                                                                |                                                            CGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCGGATGATAGCACTCGTGATCCGGGTCATA  >  NC_000913/1357596‑1357734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑