Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,374,401	Δ1 bp	coding (312/843 nt)	ycjP →	putative ABC transporter membrane subunit YcjP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,374,399	0	T	.	100.0%	72.3 / NA	18	coding (310/843 nt)	ycjP	putative ABC transporter membrane subunit YcjP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (10/8);  total (10/8)

GTCTGGTGGTGTCTGTGGTTTCATCCGTGGTGGCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGCTTTAAAGGTCGGATGACCATCAACGCCAGCTTTTACACGGTGTATATGTTCTCTGGCATTTTGCTGGTGGT  >  NC_000913/1374319‑1374469                                                                                 |                                                                       gTCTGGTGGTGTCTGTGGTTTCATCCGTGGTGGCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGCt                                                                        <  2:238741/81‑1 (MQ=255)  tCTGGTGGTGTCTGTGGTTTCATCCGTGGTGGCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGCtt                                                                       <  1:238742/81‑1 (MQ=255)         gtgtCTGTGGTTTCATCCGTGGTGGCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGC‑TTAAAGGTc                                                               <  1:238743/81‑1 (MQ=255)                tgGTTTCATCCGTGGTGGCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGC‑TTAAAGGTCGGATGAc                                                        <  1:238744/81‑1 (MQ=255)                tgGTTTCATCCGTGGTGGCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGC‑TTAAAGGTCGGATGAc                                                        >  2:238764/1‑81 (MQ=255)                       aTCCGTGGTGGCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGC‑TTAAAGGTCGGATGACCATCAAc                                                 >  2:238771/1‑81 (MQ=255)                       aTCCGTGGTGGCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGC‑TTAAAGGTCGGATGACCATCAAc                                                 >  2:238760/1‑81 (MQ=255)                           gtggtgGCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGC‑TTAAAGGTCGGATGACCATCAACGCCa                                             <  2:238745/81‑1 (MQ=255)                              gtggCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGC‑TTAAAGGTCGGATGACCATCAACGCCAGc                                           <  1:238746/80‑1 (MQ=255)                               tggCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGC‑TTAAAGGTCGGATGACCATCAACGCCAGCtt                                         >  2:238762/1‑81 (MQ=255)                                 gCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGC‑TTAAAGGTCGGATGACCATCAACGCCAGCtttt                                       <  1:238747/81‑1 (MQ=255)                                 gCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGC‑TTAAAGGTCGGATGACCATCAACGCCAGCttt                                        <  2:238748/80‑1 (MQ=255)                                          cTCGGCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGC‑TTAAAGGTCGGATGACCATCAACGCCAGCTTTTACACGgtgt                              <  2:238749/81‑1 (MQ=255)                                             ggCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGC‑TTAAAGGTCGGATGACCATCAACGCCAGCTTTTACACGGTGtata                           >  2:238780/1‑81 (MQ=255)                                               cATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGC‑TTAAAGGTCGGATGACCATCAACGCCAGCTTTTACACGGTGTATATg                         >  1:238773/1‑81 (MQ=255)                                                     tGGGGCTTACGCGCTTTCCCGCCTGCGC‑TTAAAGGTCGGATGACCATCAACGCCAGCTTTTACACGGTGTATATGTTctct                   >  2:238763/1‑81 (MQ=255)                                                              cgcgCTTTCCCGCCTGCGC‑TTAAAGGTCGGATGACCATCAACGCCAGCTTTTACACGGTGTATATGTTCTCTGGCATTTTg          >  2:238779/1‑81 (MQ=255)                                                                 gcTTTCCCGCCTGCGC‑TTAAAGGTCGGATGACCATCAACGCCAGCTTTTACACGGTGTATATGTTCTCTTGCATTTTGCtg       >  1:238777/1‑81 (MQ=255)                                                                     tCCCGCCTGCGC‑TTAAAGGTCGGATGACCATCAACGCCAGCTTTTACACGGTGTATATGTTCTCTGGCATTTTGCtggtgg   >  1:238784/1‑81 (MQ=255)                                                                       ccGCCTGCGC‑TTAAAGGTCGGATGACCATCAACGCCAGCTTTTACACGGTGTATATGTTCTCTGGCATTTTGCTggtggt  <  2:238750/80‑1 (MQ=255)                                                                                 |                                                                       GTCTGGTGGTGTCTGTGGTTTCATCCGTGGTGGCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCGCCTGCGCTTTAAAGGTCGGATGACCATCAACGCCAGCTTTTACACGGTGTATATGTTCTCTGGCATTTTGCTGGTGGT  >  NC_000913/1374319‑1374469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑