Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,476,628	(A)6→5	coding (1485/2307 nt)	ydbD →	DUF2773 domain‑containing protein YdbD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,476,623	0	A	.	100.0%	81.3 / NA	20	coding (1480/2307 nt)	ydbD	DUF2773 domain‑containing protein YdbD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (9/11);  total (9/11)

CGATGATCTAATACGTGTGTTATTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAGAAAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTAATTCACTCTGACGCTGCTTTACAACGGTTATTG  >  NC_000913/1476550‑1476697                                                                          |                                                                           cGATGATCTAATACGTGTGTTATTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAGaaaaatcc                                                                     >  1:251704/1‑78 (MQ=255)  gatgatCTAATACGTGTGTTATTTTTAGCATTGCCAGATAACTTACAGTTTAATCTTGTTAAAAGGATGGAGaaaaatccc                                                                    >  1:251701/1‑77 (MQ=255)   atgatCTAATACGTGTGTTATTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATcccc                                                                  >  1:251706/1‑81 (MQ=255)     gatCTAATACGTGTGTTATTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCgg                                                                >  1:251696/1‑81 (MQ=255)           aTACGTGTGTTATTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGcc                                                              <  1:251678/77‑1 (MQ=255)             aCGTGTGTTATTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACttt                                                        >  2:251707/1‑81 (MQ=255)              cGTGTGTTATTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACttt                                                        <  2:251679/80‑1 (MQ=255)                    ttATTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTc                                                 <  2:251680/81‑1 (MQ=255)                          ttAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGata                                           >  1:251709/1‑81 (MQ=255)                          ttAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGat                                            <  2:251681/80‑1 (MQ=255)                            aGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCGTACTTTTGCTGTCGGGATATg                                         >  2:251705/1‑81 (MQ=255)                             gCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGc                                        <  2:251682/81‑1 (MQ=255)                              cATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATg                                         >  1:251698/1‑79 (MQ=255)                                ttGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAgg                                     <  1:251683/81‑1 (MQ=255)                                  gCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGt                                    >  2:251703/1‑80 (MQ=255)                                   ccAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTaa                                  <  1:251684/81‑1 (MQ=255)                                    cAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTAAt                                 <  1:251685/81‑1 (MQ=255)                                      gATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTAATTc                               <  1:251687/81‑1 (MQ=255)                                      gATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTAATTc                               <  2:251686/81‑1 (MQ=255)                                         aaCTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTAATTCAct                            >  1:251711/1‑81 (MQ=255)                                                                   gATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTAATTCACTCTGACGCTGCTTTACAACGGTTAtt   >  2:251708/1‑80 (MQ=255)                                                                   gATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTAATTCACTCTGACGCTGCTTTACAACGGTTATTg  <  2:251688/81‑1 (MQ=255)                                                                          |                                                                           CGATGATCTAATACGTGTGTTATTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAGAAAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTAATTCACTCTGACGCTGCTTTACAACGGTTATTG  >  NC_000913/1476550‑1476697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑