Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,490,954	(T)5→4	coding (53/531 nt)	cybB →	cytochrome b561

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,490,950	0	T	.	100.0%	73.1 / NA	18	coding (49/531 nt)	cybB	cytochrome b561
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (13/5);  total (13/5)

ATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCAT  >  NC_000913/1490879‑1491023                                                                        |                                                                          aTTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGt                                                                 >  1:253424/1‑81 (MQ=255)    aTGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTAt                                                              >  2:253416/1‑81 (MQ=255)     tGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATc                                                             <  1:253399/81‑1 (MQ=255)        tAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCg                                                            >  1:253413/1‑79 (MQ=255)         aaaGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAg                                                         >  1:253418/1‑81 (MQ=255)           aGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCg                                                       >  1:253415/1‑81 (MQ=255)             cAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTa                                                     >  2:403423/1‑81 (MQ=255)              aGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTAt                                                    >  1:253414/1‑81 (MQ=255)               ggAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTAtt                                                   >  1:253419/1‑81 (MQ=255)                   aTGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTgcgc                                               <  2:253400/81‑1 (MQ=255)                              aataaataTTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTc                                    <  1:253401/81‑1 (MQ=255)                                             ttACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCAc                     <  2:253402/81‑1 (MQ=255)                                              tACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACg                    >  2:253417/1‑81 (MQ=255)                                                  aaTCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGc                >  1:253420/1‑81 (MQ=255)                                                      aGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATc            >  1:253428/1‑81 (MQ=255)                                                             aCTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTCTCTTCCCACGTAGCGATCGGCCACt     >  1:253421/1‑81 (MQ=255)                                                              ctggctggTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACtc    <  1:253403/81‑1 (MQ=255)                                                                ggctggTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACtcat  >  1:253425/1‑81 (MQ=255)                                                                        |                                                                          ATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCAT  >  NC_000913/1490879‑1491023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑