Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,519,851	(A)6→5	coding (825/1038 nt)	curA →	NADPH‑dependent curcumin/dihydrocurcumin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,519,846	0	A	.	100.0%	32.7 / NA	9	coding (820/1038 nt)	curA	NADPH‑dependent curcumin/dihydrocurcumin reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (1/8);  total (1/8)

ACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGAT  >  NC_000913/1519787‑1519922                                                            |                                                                             aCGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTG‑AAAAACGTATTCGCTTGCAAgg                                                        <  1:256938/81‑1 (MQ=255)       cAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTG‑AAAAACGTATTCGCTTGCAAGGtttt                                                    <  2:256939/79‑1 (MQ=255)        agagCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTG‑AAAAACGTATTCGCTTGCAAGGTTttatt                                                 <  2:256940/81‑1 (MQ=255)                                tttACCTCTGTTGATGGCTACAGTGCTG‑AAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCg                          >  2:256957/1‑80 (MQ=255)                                tttACCTCTGTTGATGGCTACAGTGCTG‑AAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGc                         <  2:256941/81‑1 (MQ=255)                                    ccTCTGTTGATGGCTACAGTGCTG‑AAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATc                      <  1:256943/80‑1 (MQ=255)                                    ccTCTGTTGATGGCTACAGTGCTG‑AAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATc                      <  2:256942/80‑1 (MQ=255)                                             aTGGCTACAGTGCTG‑AAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTc            <  1:256944/81‑1 (MQ=255)                                                       tGCTG‑AAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGAt  <  1:256945/81‑1 (MQ=255)                                                            |                                                                             ACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGAT  >  NC_000913/1519787‑1519922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑